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首页> 外文期刊>Plant Disease >Morphological, Biometrical, Biochemical, and Molecular Characterization of the Coffee Root-Knot Nematode Meloidogyne megadora
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Morphological, Biometrical, Biochemical, and Molecular Characterization of the Coffee Root-Knot Nematode Meloidogyne megadora

机译：咖啡根结线虫Meloidogyne megadora的形态，生物特征，生化和分子表征

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摘要

Meloidogyne megadora infects coffee trees, an economically important crop worldwide. The accurate identification of M. megadora is essential for the development of preventive measures to avoid the dispersion of this pathogen and establishment of efficient and sustainable integrated pest management programs. One M. megadora isolate was studied by biometrical, biochemical, and molecular characteristics (random amplified polymorphic DNA [RAPD] and PCR of internal transcribed spacer [ITS] region). Biometrical characteristics of M. megadora females, males, and second-stage juveniles were similar to the original description. Biochemical studies revealed a unique enzyme pattern for M. megadora esterases (Me3) that allowed for species differentiation. Three RAPD primers (OPG-4, OPG-5, and OPG-6) produced specific bands to all Meloidogyne spp. studied: M. megadora, M. arenaria, M. incognita, and M. javanica. Molecular analysis of the ITS region resulted in an amplification product of 700 bp. The phylogenetic relationship between M. megadora and several Meloidogyne spp. sequences was analyzed, revealing that M. megadora clearly differs from the most common root-knot nematode species. Based on the studies conducted, isozyme analysis remains a useful and efficient methodology for M. megadora identification when females are available. Further studies will be needed to convert the M. megadora differential DNA fragment obtained by RAPD and develop a species-specific sequence-characterized amplified region PCR assay for its diagnosis based on second-stage juveniles.
机译：巨大的Meloidogyne感染咖啡树，咖啡树是世界范围内重要的经济作物。准确鉴定巨型支原体对制定预防措施以避免这种病原体扩散以及建立有效和可持续的病虫害综合防治计划至关重要。通过生物特征，生化和分子特征（随机扩增的多态性DNA [RAPD]和内部转录间隔区[ITS]区域的PCR）研究了一种巨型分离株。巨型支原体雌性，雄性和第二阶段少年的生物特征与原始描述相似。生化研究表明，巨型支原体酯酶（Me3）具有独特的酶模式，可实现物种分化。三种RAPD引物（OPG-4，OPG-5和OPG-6）对所有Meloidogyne spp产生特异性条带。研究对象：M。megadora，M。arenaria，M。incognita和M. javanica。 ITS区域的分子分析得到700 bp的扩增产物。巨型念珠菌与几种根结线虫属之间的系统发育关系。序列分析表明，M。megadora明显不同于最常见的根结线虫物种。根据所进行的研究，当有雌性存在时，同工酶分析仍然是一种用于鉴定巨大支原体的有用且有效的方法。将需要进一步的研究来转化通过RAPD获得的巨型分枝杆菌差异DNA片段，并开发一种基于物种的特定物种的序列表征的扩增区域PCR检测方法，以基于第二阶段的幼虫进行诊断。

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