首页> 外文期刊>Plant Disease >Ratios of cells with and without virulence genes in Rhodococcus fascians populations correlate with degrees of symptom development.
【24h】

Ratios of cells with and without virulence genes in Rhodococcus fascians populations correlate with degrees of symptom development.

机译:法氏红球菌种群中有毒力基因和无毒力基因的细胞比例与症状发展程度相关。

获取原文
获取原文并翻译 | 示例
           

摘要

Rhodococcus fascians, a gram-positive phytopathogenic bacterium, causes fasciation and leafy galls on a wide range of monocotyledonous and dicotyledonous plants for which it requires the plasmid-borne fas operon. Strains isolated from symptomatic plants over a 20-year-period exhibited a high degree of variability when their virulence was assessed on garden pea seedlings. Polymerase chain reaction amplification of the fas-1 and fasR virulence genes from randomly chosen single colonies showed that many strains consisted of two subpopulations, of which one had lost these genes. Inoculation of pea seedlings with mixtures of fas-1-positive and -negative cells that originated from the same strain demonstrated a strong correlation (Pearson's r
机译:革兰氏阳性植物致病菌 Rhodococcus fascians 会在需要质粒携带的 fas 操纵子的多种单子叶植物和双子叶植物上引起迷惑和叶胆。从豌豆苗上评估其毒力后,从有症状植物中分离出的菌株在20年以上的时间内表现出高度的变异性。随机选择的单个菌落中 fas-1 和 fasR 毒力基因的聚合酶链反应扩增显示,许多菌株由两个亚群组成,其中一个失去了这些基因。豌豆幼苗接种源自同一菌株的 fas-1 阳性细胞和-阴性细胞的混合物显示出比例之间的强相关性(Pearson's 基因的接种物中的细胞数量和疾病症状的严重程度。豌豆幼苗上小的侧生芽发育所需的 fas-1 阳性细胞的最低浓度为2.5x10 4 CFU / ml( P <= 0.008),而在2.5x10 5 CFU / ml( P <= 0.019)处观察到主茎生长的总体抑制。这些观察结果强调,当临床R的毒力时应注意。法西斯人的菌株进行了评估。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号