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首页> 外文期刊>Plant Disease >First report of tomato torrado virus infecting tomato in Hungary.
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First report of tomato torrado virus infecting tomato in Hungary.

机译：在匈牙利，番茄龙卷风病毒感染番茄的首次报道。

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During the growing seasons of 2007 and 2008, in commercial greenhouses of tomato in Szeged, Ocsod and Csongrad (Hungary), unusual disease symptoms were observed, including necrotic spots in defined areas at the base of the leaflet, necrosis in the stems, and necrotic lines on the fruit surface. Affected plants appeared inside the greenhouses with a random distribution and the incidence recorded was at least 40%. These symptoms resembled those described for Tomato torrado virus (ToTV) infection in Spain and Poland. To verify the identity of the disease, 3 symptomatic plants from commercial greenhouses of each geographical location were selected and analysed by DAS-ELISA using polyclonal antibodies specific to Cucumber mosaic virus (CMV), Potato virus Y (PVY), Tomato mosaic virus (ToMV), Tomato spotted wilt virus (TSWV) and Pepino mosaic virus (PepMV). All the 3 samples were positive for ToTV by molecular hybridization and RT-PCR. The RT-PCR products were purified and directly sequenced. The amplified fragments of the 3 Hungarian isolates, ToTV-H1, ToTV-H2 and ToTV-H3, for the polyprotein (GenBank Accession Nos. EU835496, FJ616995 and FJ616994, respectively) and the CP Vp23 (GenBank Accession Nos. FJ616996, FJ616997 and FJ 616998, respectively) showed 99-98% nt identity with the polyprotein and the coat protein regions of ToTV from Spain and Poland (GenBank Accession Nos. DQ3888880 and EU563947, respectively). Whiteflies, commonly found in Hungarian greenhouses, have been reported to transmit ToTV, although the efficiency of transmission is unknown. This is thought to be the first report of ToTV in Hungary.
机译：在2007年和2008年的生长季节中，在塞格德，Ocsod和Csongrad（匈牙利）的商业番茄大棚中，观察到异常的疾病症状，包括小叶底部特定区域的坏死斑，茎部坏死和坏死。水果表面上的线条。受影响的植物以随机分布出现在温室内，记录的发病率至少为40％。这些症状类似于在西班牙和波兰描述的“番茄龙卷风病毒”（ToTV）感染的症状。为了验证该疾病的身份，从每个地理位置的商业温室中选择了3株有症状的植物，并使用针对黄瓜花叶病毒（CMV）和马铃薯病毒的多克隆抗体，通过DAS-ELISA分析Y （PVY），番茄花叶病毒（ToMV），番茄斑萎病毒（TSWV）和 Pepino花叶病毒（PepMV ）。通过分子杂交和RT-PCR，所有3个样品的ToTV均为阳性。纯化RT-PCR产物并直接测序。 3个匈牙利分离株ToTV-H1，ToTV-H2和ToTV-H3的扩增片段，分别用于多蛋白（GenBank登录号EU835496，FJ616995和FJ616994）和CP Vp23（GenBank登录号FJ616996，FJ616997和FJ 616998分别与西班牙和波兰的ToTV的多蛋白和外壳蛋白区域显示了99-98％nt的同一性（GenBank登录号分别为DQ3888880和EU563947）。据报道，通常在匈牙利温室中发现的粉虱可以传播ToTV，尽管其传播效率尚不清楚。这被认为是匈牙利ToTV的第一份报道。

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