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An Evaluation of the Flora Adjacent to Wine Grape Vineyards for the Presence of Alternative Host Plants of Grapevine red blotch-associated virus

机译:葡萄葡萄园附近植物区系存在与葡萄红斑病相关病毒的替代寄主植物的评估

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摘要

Grapevine red blotch-associated virus (GRBaV) is a recently discovered virus of concern to wine grape production in North America. While the vector of this virus is unknown, other elements of virus epidemiology are essential to develop guidelines forthe management of the virus as well as to assist in the search for its vector. The objective of this study was to evaluate vegetation within and surrounding GRBaV-infected vineyards to identify potential virus reservoirs that may serve as sources of inoculum. In this study, 13 plant species were sampled throughout the year and weretested for the presence of GRBaV. Of the 13 species tested, two species, Rubus armeniacus and wild grapes (Vitis californica x V. vinifera), tested positive by quantitative PCR. Of these two species, only wild grapes were determined to be a true host. This study documents the first time GRBaV has been confirmed in an alternative host or in a species outside of a commercial vineyard and suggests that a mechanism exists by which GRBaV moves between plant species that is not human-mediated. The precise role that wild grapes play in the epidemiology of GRBaV remains unknown.
机译:葡萄红斑病相关病毒(GRBaV)是最近发现的与北美酿酒葡萄有关的病毒。虽然该病毒的载体未知,但病毒流行病学的其他要素对于开发病毒管理指南以及协助寻找其载体至关重要。这项研究的目的是评估受GRBaV感染的葡萄园内和周围的植被,以识别可能作为接种源的潜在病毒库。在这项研究中,全年对13种植物进行了采样,并测试了GRBaV的存在。在所测试的13个物种中,有两个物种,即悬钩子和野生葡萄(Vitis californica x V. vinifera),通过定量PCR呈阳性。在这两个物种中,只有野生葡萄才被确定为真正的寄主。这项研究记录了GRBaV首次在其他寄主中或在商业葡萄园以外的物种中被证实,并表明存在GRBaV在非人类介导的植物物种之间移动的机制。野生葡萄在GRBaV流行病学中所起的确切作用仍然未知。

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