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First report of cucurbit chlorotic yellows virus infecting melon in China.

机译：葫芦科黄萎病病毒在中国的首次报道。

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In October 2007, symptoms of chlorosis on the upper leaves and a bright yellow colour on the lower leaves were observed sporadically on hami melon (Cucumis melo cv. Xuelihong) in a high tunnel in Nanhui, Shanghai, China. Disease progressed from initial mottling of leaves into leaves that were completely yellow with the veins remaining green. The oldest leaves developed symptoms first, so these leaves had a pronounced even yellow colour. In October 2009, these symptoms were found in all melons produced in the suburbs of Shanghai. Twelve samples from symptomatic melons were collected in the Jiading, Nanhui, Fengxian and Chongming districts of Shanghai for virus diagnosis. Large populations of whiteflies were observed in association with the diseased cucurbit crops. Total RNA was extracted and random primers were used for RT-PCR. The expected 462 bp target fragment of Cucurbit chlorotic yellows virus (CCYV) was obtained in all infected samples. All the 462 bp PCR products were cloned and sequenced. All sequences obtained were homologous. A comparison of the submitted sequence with those in GenBank showed that the sequence had 100% nucleotide identity to the Hsp70h sequences of CCYV isolates from Japan, Taiwan and mainland of China. This is thought to be the first report of CCYV on melon in China.Digital Object Identifier http://dx.doi.org/10.1094/PDIS-08-10-0613
机译：2007年10月，在上海南汇高地隧道的哈密瓜（ cv。Xuelihong）上偶发地观察到上部叶子发生绿化症状，下部叶子出现亮黄色。 。疾病从最初的叶子斑驳发展成完全黄色的叶子，而叶脉仍保持绿色。最老的叶子​​首先出现症状，因此这些叶子具有明显的甚至黄色。 2009年10月，在上海郊区生产的所有瓜中都发现了这些症状。在上海的嘉定区，南汇区，奉贤区和崇明区采集了十二种有症状瓜类样品，用于病毒诊断。观察到大量粉虱与患病的葫芦科作物有关。提取总RNA，并将随机引物用于RT-PCR。在所有感染的样品中均获得了预期的462 bp的葫芦绿黄病毒（CCYV）靶片段。克隆所有462 bp PCR产物并测序。获得的所有序列都是同源的。将提交的序列与GenBank中的序列进行比较表明，该序列与来自日本，台湾和中国大陆的CCYV分离株的Hsp70h序列具有100％的核苷酸同一性。这被认为是CCYV在中国的第一份关于瓜的报告。数字对象标识符http://dx.doi.org/10.1094/PDIS-08-10-0613

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