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首页> 外文期刊>Plant Disease >Nondefoliating and Defoliating Strains from Cotton Correlate with Races 1 and 2 of Verticillium dahliae
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Nondefoliating and Defoliating Strains from Cotton Correlate with Races 1 and 2 of Verticillium dahliae

机译：棉花的脱叶和脱叶菌株与黄萎病的第1和第2种族相关

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Verticillium wilt, caused by Verticillium dahliae, is an important disease of cotton worldwide. Isolates of V. dahliae can be characterized as race 1 or race 2 based on the responses of differential cultivars of tomato and lettuce, or as defoliating or nondefoliating based on symptom expression in cotton. To investigate the frequency and distribution of races and defoliation phenotypes of cotton-associated V. dahliae, 317 isolates from China, Israel, Turkey, and the United States were tested by polymerase chain reaction (PCR) using defoliating, nondefoliating, and race 1- and race 2-specific primers DF/DR, NDF/NDR, VdAve1F/VdAve1R, and VdR2F/VdR2R, respectively. Of the total, 97.2% of isolates genotyped as defoliating were also characterized as race 2, while 90.8% of isolates genotyped as nondefoliating were also genotyped as race 1. To verify these results, three cotton cultivars -'FM 2484B2F' (highly resistant), '98M-2983' (highly susceptible), and 'CA4002' (partially resistant)-used as differentials were each inoculated with 10 isolates characterized by PCR: six defoliating/race 2 strains (GH1005, GH1021, FIN, XJ2008, XJ592, and reference strain Ls17) and four nondefoliating/race 1 strains (GH1015, GH1016, GH1020, and reference strain Ls16). All defoliating/race 2 isolates except for Ls17 caused defoliation on 98M-2983 and CA4002. Isolate Ls17 caused defoliation on 98M-2983 only. The nondefoliating/race 1 isolates caused Verticillium wilt symptoms devoid of defoliation on 98M-2983. The greenhouse assays confirmed the molecular identification of race and defoliation phenotype. Although the existence of races has not been previously established among V. dahliae isolates from cotton, the long-established nondefoliating and defoliating population structure corresponded with V. dahliae races 1 and 2, respectively.
机译：黄萎病引起的黄萎病是全世界棉花的重要病害。基于番茄和生菜的不同栽培品种的反应，大丽弧菌的分离株可表征为第1种族或第2种族，或者基于棉花的症状表达可表征为脱叶或无叶。为了调查棉花相关大丽花V的种族和脱叶表型的频率，分布，中国，以色列，土耳其和美国的317个分离株，采用脱叶，不脱叶和第1种族通过聚合酶链反应（PCR）进行了测试。和种族2特异性引物DF / DR，NDF / NDR，VdAve1F / VdAve1R和VdR2F / VdR2R。在总数中，有97.2％的基因型为落叶的菌株也属于第2种，而有90.8％的基因型为无落叶的菌株也有第1种的基因型。为验证这些结果，使用了三个棉花品种'FM 2484B2F'（高抗性） ，“ 98M-2983”（高度易感）和“ CA4002”（部分抗性）用作差异，分别接种了10个以PCR为特征的分离株：6个落叶/种族2菌株（GH1005，GH1021，FIN，XJ2008，XJ592，和参考菌株Ls17）和四个非脱叶/种族1菌株（GH1015，GH1016，GH1020和参考菌株Ls16）。除Ls17外的所有脱叶/种族2分离株均对98M-2983和CA4002造成脱叶。分离株Ls17仅在98M-2983上引起脱叶。非脱叶/种族1分离株在98M-2983上引起黄萎病症状，但没有脱叶现象。温室试验证实了种族和落叶表型的分子鉴定。尽管以前尚未在棉花的大丽花弧菌分离株中建立种族，但早已建立的不脱叶和落叶的种群结构分别对应于大丽花弧菌1和2。

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