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首页> 外文期刊>Plant Disease >Characterization of Beet curly top virus Strains Circulating in Beet Leafhoppers (Hemiptera: Cicadellidae) in Northeastern Oregon
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Characterization of Beet curly top virus Strains Circulating in Beet Leafhoppers (Hemiptera: Cicadellidae) in Northeastern Oregon

机译：在俄勒冈州东北的甜菜叶蝉（Hemiptera：Cicadellidae）中传播的甜菜卷曲顶病毒菌株的鉴定。

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摘要

The beet leafhopper, Circulifer tenellus, is an agriculturally important pest, particularly in the western United States. This insect transmits the Beet curly top virus (BCTV) to multiple crops, including bean, tomato, and pepper. In this study, we investigated the incidence of BCTV in individual leafhoppers collected at several sites in northeastern Oregon during the growing season in 2007, 2008, and 2009. Of the 800 insects tested, 151 (18.9%) were found positive for the virus. Percentage of virus incidence varied from 0% at one location in 2009 to a high of 55.6% for a location sampled in 2008. The complete virus genomes from one virus-positive insect collected in each of the 3 years were determined. BLAST analysis of the BCTV whole-genome sequences from 2007, 2008, and 2009 insects showed 98, 94, and 96% identities with the BCTV-Worland sequence (AY134867), respectively. The BCTV_2008 sequence showed the greatest identity (96%) with another BCTV genomic sequence (JN817383), and was found to be a recombinant between the BCTV-Worland type, representing the majority of the genome (approximately 2.2 kb), and the BCTV-CFH type that provided an approximately 0.8-kb fragment spanning replication-related genes Cl and C2. This area of the BCTV genome, between the Cl and C2 genes, was previously found to carry symptom determinants of the virus, and the data may suggest more severe effects of BCTV during the 2008 season. Results indicate that BCTV is common and widespread in C. tenellus in eastern Oregon and that there is substantial genetic diversity among the virus strains present in this important field and vegetable crop-growing region.
机译：甜菜叶蝉，Circulifer tenellus，是一种重要的农业害虫，在美国西部尤其如此。这种昆虫将甜菜卷曲顶病毒（BCTV）传播到多种农作物，包括豆类，番茄和胡椒。在这项研究中，我们调查了2007、2008和2009年生长季节在俄勒冈州东北部几个地点收集的单个叶蝉的BCTV发生率。在测试的800种昆虫中，有151例（18.9％）被发现对该病毒呈阳性。病毒发生率的百分比从2009年的一个位置的0％到2008年采样的一个位置的55.6％的高位。确定了在3年中每年收集的来自一种病毒阳性昆虫的完整病毒基因组。对2007年，2008年和2009年昆虫的BCTV全基因组序列进行的BLAST分析显示，与BCTV-Worland序列（AY134867）的同源性分别为98％，94％和96％。 BCTV_2008序列与另一个BCTV基因组序列（JN817383）表现出最大的同一性（96％），并且被发现是BCTV-Worland类型（代表基因组的大部分（约2.2 kb））和BCTV- CFH类型提供了一个大约0.8kb的片段，跨越复制相关基因C1和C2。先前发现BCTV基因组的这一区域位于C1和C2基因之间，带有病毒的症状决定因素，并且该数据可能表明BCTV在2008年季节期间的影响更为严重。结果表明，BCTV在俄勒冈州东部的C. tenellus中很常见，并且很普遍，并且在这个重要田地和蔬菜作物种植地区存在的病毒株之间存在大量遗传多样性。

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