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首页> 外文期刊>Plant Disease >Use of Mycelium and Detached Leaves in Bioassays for Assessing Resistance to Boxwood Blight
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Use of Mycelium and Detached Leaves in Bioassays for Assessing Resistance to Boxwood Blight

机译：在生物测定中使用菌丝体和分离的叶子评估黄杨枯萎病的抗性

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摘要

Boxwood blight caused by Calonectria pseudonaviculata is a newly emergent disease of boxwood (Buxus spp. L.) in the United States that causes leaf drop, stem lesions, and plant death. A rapid and reliable laboratory assay that enables screening hundreds of boxwood genotypes for resistance to boxwood blight is needed to enable breeding and selection of resistant cultivars. Using eight boxwood cultivars with differing susceptibilities, we examined parameters for a screening assay comparing whole plant inoculation with detached leaf inoculation, use of mycelium versus spores as the inoculum, comparison of times of the year for inoculation, and comparison of two leaf inoculation methods. Inoculation of detached leaves gave comparable results to inoculation of whole plants when compared across genotypes, although the detached leaf assay resulted in greater percentages of symptom expression. The time of year of plant inoculation (spring, summer, or winter) did not affect the relative expression of symptoms among the most resistant and susceptible genotypes. Inoculating plants with mycelium was as effective as spore inoculation for causing disease symptoms and allowed us to distinguish the more resistant genotypes, yet mycelium inoculation was much easier to prepare in large quantities for multiple assays.
机译：由拟南芥（Calonectria pseudonaviculata）引起的黄杨枯萎病是在美国出现的一种新近出现的黄杨病（Buxus spp。L.），引起叶片掉落，茎部损伤和植物死亡。需要一种快速可靠的实验室测定方法，该方法能够筛选出数百种黄杨木型对黄杨枯萎病的抗性，以便进行育种和选择抗病品种。我们使用八个敏感性不同的黄杨木栽培品种，检查了用于筛选试验的参数，该试验比较了整株接种与离体接种的接种，菌丝体对孢子的接种，一年中接种次数的比较以及两种接种方法的比较。跨基因型比较，分离叶的接种与整株植物接种的结果相当，尽管分离叶试验可导致更大百分比的症状表达。一年中接种植物的时间（春季，夏季或冬季）不影响最抗病和易感基因型中症状的相对表达。用菌丝体接种植物与引起疾病症状的孢子接种一样有效，并且使我们能够区分更具抗性的基因型，但菌丝体接种更易于大量制备用于多种测定。

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