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首页> 外文期刊>Plant physiology >Microarray analysis of the genome-wide response to iron deficiency and iron reconstitution in the cyanobacterium Synechocystis sp PCC 6803
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Microarray analysis of the genome-wide response to iron deficiency and iron reconstitution in the cyanobacterium Synechocystis sp PCC 6803

机译：基因芯片对蓝藻集胞藻PCC 6803中铁缺乏和铁重构的全基因组反应的微阵列分析

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A full-genome microarray of the (oxy)photosynthetic cyanobacterium Synechocystis sp. PCC 6803 was used to identify genes that were transcriptionally regulated by growth in iron (Fe)-deficient versus Fe-sufficient media. Transcript accumulation for 3,165 genes in the genome was analyzed using an analysis of variance model that accounted for slide and replicate (random) effects and dye (a fixed) effect in testing for differences in the four time periods. We determined that 85 genes showed statistically significant changes in the level of transcription (P less than or equal to 0.05/3,165 = 0.0000158) across the four time points examined, whereas 781 genes were characterized as interesting (P less than or equal to 0.05 but greater than 0.0000158; 731 of these had a fold change >1.25X). The genes identified included those known previously to be Fe regulated, such as isiA that encodes a novel chlorophyll-binding protein responsible for the pigment characteristics of low-Fe (LoFe) cells. ATP synthetase and phycobilisome genes were down-regulated in LoFe, and there were interesting changes in the transcription of genes involved in chlorophyll biosynthesis, in photosystem I and II assembly, and in energy metabolism. Hierarchical clustering demonstrated that photosynthesis genes, as a class, were repressed in LoFe and induced upon the re-addition of Fe. Specific regulatory genes were transcriptionally active in LoFe, including two genes that show homology to plant phytochromes (cph1 and cph2). These observations established the existence of a complex network of regulatory interactions and coordination in response to Fe availability.
机译：（氧）光合蓝藻Synechocystis sp。的全基因组微阵列。 PCC 6803用于鉴定在缺乏铁（Fe）的培养基中与缺乏铁的培养基中的生长相关的转录调控基因。使用方差分析模型分析了基因组中3,165个基因的转录物积累，该模型在测试四个时间段的差异时考虑了滑动和复制（随机）效应和染料（固定）效应。我们确定，在检查的四个时间点上，有85个基因在转录水平上显示出统计学上的显着变化（P小于或等于0.05 / 3,165 = 0.0000158），而781个基因被表征为有趣的（P小于或等于0.05，但大于0.0000158;其中731的倍数变化大于1.25倍。鉴定出的基因包括以前已知受Fe调控的基因，例如isiA，其编码负责低Fe（LoFe）细胞色素特性的新型叶绿素结合蛋白。 LoFe中的ATP合成酶和藻胆体基因被下调，并且参与叶绿素生物合成，光系统I和II装配以及能量代谢的基因的转录发生了有趣的变化。层次聚类表明，光合作用基因作为一类在LoFe中受到抑制，并在重新添加Fe时被诱导。特定的调控基因在LoFe中具有转录活性，其中包括两个与植物光色素具有同源性的基因（cph1和cph2）。这些发现建立了响应铁可用性的复杂的监管相互作用和协调网络。

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