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Identification and comparative analysis of low phosphate tolerance-associated microRNAs in two maize genotypes

机译：两种基因型与低磷酸盐耐性相关的微小RNA的鉴定和比较分析

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摘要

Low phosphate (Pi) availability is a major constraint on maize growth and productivity. MicroRNAs (miRNAs) are known to play critical roles in plant responses to various environmental conditions. The identification of low Pi tolerance-associated miRNAs will accelerate the development of Pi starvation tolerant maize plants. However, miRNAs associated with low Pi tolerance have not been identified. In this study, we compared deep sequencing small RNA reads from two maize genotypes, the wild type, Qi319, and the low Pi tolerant mutant, 99038, under normal and low Pi conditions. Six known miRNA families and seven novel miRNAs were found differently expressed by the two genotypes. All these miRNAs were confirmed by sequencing a second batch of small RNA libraries constructed in the same way as those used in the first sequencing. The expression profiles of some of these miRNAs were further confirmed by real-time PCR. The predicted target genes of the low Pi tolerance-associated miRNAs were involved in root development or stress responses. Expression levels of some of target genes were significantly different between Qi319 and 99038. These findings suggested that miRNAs may play important roles in low Pi tolerance in maize and may be a key factor in determining the level of low Pi tolerance in different maize genotypes. This study provides an approach for identifying low Pi tolerance-associated miRNAs and can help in the selection and manipulation of high performing maize genotypes under low Pi conditions.
机译：磷（Pi）利用率低是玉米生长和生产力的主要限制因素。已知MicroRNA（miRNA）在植物对各种环境条件的反应中起关键作用。与低Pi耐受性相关的miRNA的鉴定将加速耐Pi饥饿的玉米植物的发育。但是，尚未鉴定出与低Pi耐受性相关的miRNA。在这项研究中，我们比较了在正常和低Pi条件下从两种玉米基因型野生型Qi319和低Pi耐性突变体99038进行的小RNA读取的深度测序。发现两个基因型以不同的方式表达了六个已知的miRNA家族和七个新颖的miRNA。通过对第二批小RNA文库进行测序来确认所有这些miRNA，这些小RNA文库的构建方式与第一次测序中所用的相同。通过实时PCR进一步证实了其中一些miRNA的表达谱。低Pi耐受性相关的miRNA的预测目标基因参与了根发育或胁迫反应。 Qi319和99038之间某些靶基因的表达水平显着不同。这些发现表明，miRNA可能在玉米的低磷耐受性中起重要作用，并且可能是决定不同玉米基因型低磷耐受性水平的关键因素。这项研究提供了一种鉴定低Pi耐受性相关miRNA的方法，并有助于在低Pi条件下选择和操纵高性能玉米基因型。

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