...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant Physiology and Biochemistry >Identification of miRNAs involved in long-term simulated microgravity response in Solanum lycopersicum
【24h】

Identification of miRNAs involved in long-term simulated microgravity response in Solanum lycopersicum

机译：番茄中长期参与模拟微重力反应的miRNA的鉴定

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To identify the miRNAs associated with the simulated microgravity response in plants and to ascertain the regulation network mediated by miRNAs under simulated microgravity conditions, we constructed a miRNA library by direct cloning method and analyzed the library. Seven miRNAs that are conserved in other plants were cloned for the first time in Solanum lycopersicum under simulated microgravity condition. The expressions of six of the seven miRNAs were up-regulated, especially by long-term simulated microgravity. Gene ontology analysis showed that most of the predicted targeted genes were involved in transcription regulation, signal transduction and stress response, implying a complicated relationship among the external signal, internal transduction and final phenotype. Six of the predicted targets were validated by 5' RACE and reverse transcription real-time quantitative PCR. The results showed that with increasing miRNA expression levels, the corresponding target genes were down-regulated. The target gene of one of miRNAs, miR159e*, was thought to be associated with an increasing of starch amount under microgravity condition. A multi-stresses response network mediated by miRNAs under simulated microgravity condition was proposed. Cis-elements located in the upstream sequences of each miRNA were identified and their roles in gene regulation were investigated. In addition to the seven miRNAs that had homologs in other plants, six conserved S lycopersicum miRNAs were identified. In the study, miRNAs were identified in S. lycopersicum for the first time under long-term simulated microgravity condition, which will help reveal the regulation mechanism mediated by miRNAs under simulated microgravity condition and adaptation to Earth's gravity.
机译：为了鉴定与植物中模拟微重力反应相关的miRNA，并确定在模拟微重力条件下miRNA介导的调控网络，我们通过直接克隆方法构建了miRNA文库并对其进行了分析。在模拟微重力条件下，首次在其他植物中首次克隆了七个在其他植物中保守的miRNA。七个miRNA中的六个的表达被上调，尤其是通过长期模拟的微重力。基因本体分析表明，大多数预测的靶基因都参与转录调控，信号转导和应激反应，这暗示了外部信号，内部转导和最终表型之间的复杂关系。通过5'RACE和逆转录实时定量PCR验证了六个预测目标。结果表明，随着miRNA表达水平的提高，相应的靶基因被下调。 miRNA之一的miR159e *的靶基因被认为与微重力条件下淀粉含量的增加有关。提出了在模拟微重力条件下由miRNAs介导的多应力反应网络。确定了位于每个miRNA上游序列中的顺式元件，并研究了它们在基因调控中的作用。除了在其他植物中具有同源性的七个miRNA，还鉴定了六个保守的S lycopersicum miRNA。在这项研究中，在长期模拟微重力条件下首次在番茄链球菌中鉴定了miRNA，这将有助于揭示在模拟微重力条件下miRNA介导的调控机制以及对地球重力的适应性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号