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首页> 外文期刊>Plant Physiology and Biochemistry >Isolation and developmental expression analysis of L-myo-inositol-1-phosphate synthase in four Actinidia species
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Isolation and developmental expression analysis of L-myo-inositol-1-phosphate synthase in four Actinidia species

机译：四种猕猴桃中L-肌-肌醇-1-磷酸合酶的分离和发育表达分析

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摘要

Myo-inositol (MI) is an important polyol involved in cellular signal transduction, auxin storage, osmotic regulation, and membrane formation. It also serves as a precursor for the production of pinitol, ascorbic acid, and members of the raffinose family. The first committed step for MI formation is catalyzed by Lmyo- inositol-1-phosphate synthase (MIPS). We isolated MIPS cDNA sequences from Actinidia eriantha, Actinidia rufa, and Actinidia arguta and compared them with that of Actinidia deliciosa. Each comprised 1533 bp, encoding 510 amino acids with a predicted molecular weight of 56.5 KDa. The MIPS protein was highly conserved in Actinidia, sharing 98.94% identity among species. The MIPS gene was expressed in the flowers, leaves, petioles, and carpopodia. Similarly high levels of expression were detected in the young fruit of all four species. Overall activity of the enzyme was also maximal in young fruit, indicating that this developmental stage is the key point for MI synthesis in Actinidia. Among the four species, A. arguta had the greatest concentration of MI as well as the highest ratios of MI:sucrose and MI:glucose + fructose. This suggests that conversion to MI from carbohydrates was most efficient in A. arguta during early fruit development.
机译：肌醇（MI）是一种重要的多元醇，参与细胞信号转导，生长素存储，渗透调节和膜形成。它还可用作生产松油醇，抗坏血酸和棉子糖家族成员的前体。 MI形成的第一步是Lmyo-肌醇-1-磷酸合酶（MIPS）。我们从猕猴桃，猕猴桃和紫花猕猴桃中分离出MIPS cDNA序列，并将其与猕猴桃进行了比较。每个包含1533 bp，编码510个氨基酸，预测分子量为56.5 KDa。 MIPS蛋白在猕猴桃中高度保守，在种间共有98.94％的同一性。 MIPS基因在花，叶，叶柄和卡波菌中表达。同样，在所有四个物种的幼果中也检测到高水平的表达。该酶的整体活性在幼果中也最大，表明该发育阶段是猕猴桃中MI合成的关键点。在这四个物种中，A。arguta的MI浓度最高，而MI：蔗糖和MI：葡萄糖+果糖的比例最高。这表明在早期果实发育过程中，从碳水化合物转化成MI在arguta arguta中最有效。

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