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首页> 外文期刊>Plant Physiology and Biochemistry >Proteomic analysis of ' Moncada' mandarin leaves with contrasting fruit load
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Proteomic analysis of ' Moncada' mandarin leaves with contrasting fruit load

机译：“ Moncada”普通话叶片的蛋白质组学分析与相反的水果负荷

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摘要

A proteomic approach was used to know more about the molecular mechanism related to Citrus alternate bearing. To this end, we researched protein expression differences between on-crop and off-crop " Moncada" [Clementine 'Oroval' (Citrus clementina Hort ex Tanaka) x 'Kara' mandarin (Citrus unshiu Marc. x Citrus nobilis Lou.)] mandarin leaves. This variety usually shows a remarkable behaviour in alternate production. Samples were collected in the period during which the fruit affect flowering induction. From 2D DIGE gel, 110 spots were isolated: 43 showed increased expression in the off-crop samples compared to on-crop samples, while 67 showed increased expression in the on-crop samples against off-crop samples. These spots were identified by MALDI-MS or LC-MS-MS. According to the up-expressed proteins in off-crop leaves such as proteins related to nutrient reservoir activity or to the pentose phosphate pathway, the primary metabolism was more active in off-crop trees than in on-crop trees. In contrast, the proteins up-expressed in on-crop samples such as catalase were related to the oxidoreductase activity and, therefore, the redox state seemed different for off-crop and for on-crop leaves. Other proteins with unknown functions were isolated, which could be also related to the alternate bearing and to the flowering induction.
机译：蛋白质组学方法被用来了解有关柑橘交替轴承的分子机制的更多信息。为此，我们研究了农作物和农作物“ Moncada” [柑桔'Oroval（柑桔柑桔Hor ex Tanaka）x'Kara'柑桔（柑桔柑桔x柑桔tru）的蛋白质表达差异。树叶。该品种通常在替代生产中表现出显着的行为。在果实影响开花诱导的时期内收集样品。从2D DIGE凝胶中分离出110个斑点：与农作物样品相比，农作物样品中43个蛋白的表达增加，而农作物样品中67个蛋白在农作物样品中的表达增加。这些斑点通过MALDI-MS或LC-MS-MS鉴定。根据非农作物叶片中高表达的蛋白质（例如与养分库活性或磷酸戊糖途径相关的蛋白质），非农作物树木中的主要代谢活性高于非农作物树木。相反，在农作物样品中过表达的蛋白质（如过氧化氢酶）与氧化还原酶活性相关，因此，农作物和农作物叶片的氧化还原状态似乎不同。分离出功能未知的其他蛋白质，这也可能与备用轴承和开花诱导有关。

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