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首页> 外文期刊>Plant Science: An International Journal of Experimental Plant Biology >Nitrate regulation of the oxidative pentose phosphate pathway in maize (Zea mays L.) root plastids: induction of 6-phosphogluconate dehydrogenase activity, protein and transcript levels.
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Nitrate regulation of the oxidative pentose phosphate pathway in maize (Zea mays L.) root plastids: induction of 6-phosphogluconate dehydrogenase activity, protein and transcript levels.

机译：玉米（Zea mays L.）根质体中氧化性戊糖磷酸途径的硝酸盐调节：诱导6-磷酸葡萄糖酸脱氢酶活性，蛋白质和转录水平。

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摘要

The effect of nitrate on the expression of the NADPH producing enzymes of the oxidative pentose phosphate pathway, glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) was examined in maize seedlings (Zea mays, W64AX A182E). In extracts of 5-day-old maize roots and leaves treated with 10 mM KNO3, G6PDH and 6PGDH activities increased by 44 and 53%, respectively, relative to untreated roots. In isolated plastids from KNO3 treated roots, G6PDH and 6PGDH specific activities were more than 25- and 12-fold higher than in the untreated control. Western blot analysis showed higher levels of 6PGDH protein in root plastid extracts from KNO3 treated plants. The data suggest that KNO3 specifically affects the plastidic formsof G6PDH and 6PGDH. Three classes of 6PGDH cDNA were identified in maize roots. Of these, one cDNA hybridized with a transcript that accumulated rapidly and transiently in response to low concentrations of external nitrate. The accumulation of this transcript was not affected by pretreating plants with 50μg/ml cycloheximide, which was previously shown to inhibit cytoplasmic protein synthesis in maize roots by more than 85%. Neither NH+4 nor K+ treatment affected transcript accumulation. The data indicate coordinated regulation of genes and enzymes required for NO-3 assimilation and NADPH production in root plastids.
机译：在玉米幼苗中（Zea mays，W64AX A182E）检查了硝酸盐对氧化戊糖磷酸途径的NADPH产生酶，葡萄糖6-磷酸脱氢酶（G6PDH）和6-磷酸葡萄糖酸脱氢酶（6PGDH）表达的影响。在用10 mM KNO3处理的5天龄玉米根和叶提取物中，相对于未经处理的根，G6PDH和6PGDH活性分别提高了44％和53％。在从KNO3处理过的根中分离出的质体中，G6PDH和6PGDH的比活性比未处理的对照高25倍和12倍。蛋白质印迹分析显示，来自KNO3处理过的植物的根质提取物中6PGDH蛋白含量较高。数据表明，KNO3特异性影响G6PDH和6PGDH的质体形式。在玉米根中鉴定出三类6PGDH cDNA。其中的一个cDNA与转录物杂交，该转录物响应低浓度的外部硝酸盐而迅速且短暂地积累。用50μg/ ml的环己酰亚胺对植物进行预处理不会影响该转录本的积累，此前已证明该环糊精可抑制玉米根部细胞质蛋白的合成超过85％。 NH + 4和K +处理均不影响转录本的积累。数据表明根质体中NO-3同化和NADPH产生所需的基因和酶的协调调节。

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