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首页> 外文期刊>Plant Science: An International Journal of Experimental Plant Biology >Tissue-specific expression in transgenic rice and Arabidopsis thaliana plants of GUS gene driven by the 5' regulatory sequences of an anther specific rice gene YY2
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Tissue-specific expression in transgenic rice and Arabidopsis thaliana plants of GUS gene driven by the 5' regulatory sequences of an anther specific rice gene YY2

机译：花药特异性水稻基因YY2 5'调控序列驱动GUS基因在转基因水稻和拟南芥植物中的组织特异性表达

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摘要

The identification and characterization of promoters, cis-elements and transcription factors are critical for studying gene expression during growth and development in any organism. Use of specific promoters is an absolute requirement for the expression of foreign genes in plants in a developmentally, spatially and/or temporally regulated manner. The YY2 cDNA has been shown to be expressed specifically in the tapetum cells of rice anther. In this study, a 968 bp upstream regulatory sequence (GenBank accession no. FJ957881) of the gene was isolated using genome walking method. The isolated fragment was shown to drive anther-specific expression of GUS reporter gene in rice as well as in the dicot plant Arabidopsis thaliana. Deletion analysis revealed that a [not, vert, similar]400 bp 5' regulatory region is sufficient to confer anther-specific expression of GUS gene.
机译：启动子，顺式元件和转录因子的鉴定和表征对于研究任何生物在生长和发育过程中的基因表达至关重要。特定启动子的使用是植物中外源基因以发育，空间和/或时间调控方式表达的绝对要求。已显示YY2 cDNA在水稻花药的绒毡层细胞中特异性表达。在这项研究中，使用基因组步移方法分离了该基因的968 bp上游调控序列（GenBank登录号FJ957881）。分离的片段显示出在水稻以及双子叶植物拟南芥中驱动GUS报告基因的花药特异性表达。缺失分析显示[非，垂直，相似] 400 bp 5'调节区足以赋予GUS基因花药特异性表达。

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