...
首页> 外文期刊>Plant Science: An International Journal of Experimental Plant Biology >Characterization of three linalool synthase genes from Citrus unshiu Marc. and analysis of linalool-mediated resistance against Xanthomonas citri subsp citri and Penicilium italicum in citrus leaves and fruits
【24h】

Characterization of three linalool synthase genes from Citrus unshiu Marc. and analysis of linalool-mediated resistance against Xanthomonas citri subsp citri and Penicilium italicum in citrus leaves and fruits

机译:来自柑桔的三个芳樟醇合酶基因的表征。芳醇介导的柑桔叶片和果实中柠檬黄单胞菌和柠檬青霉的抗药性分析

获取原文
获取原文并翻译 | 示例
           

摘要

Three cDNA clones from Satsuma mandarin (Citrus unshiu Marc.) were isolated and expressed in Escherichia coil. CuSTS3-1 and CuSTS3-2 encode linalool synthases and CuSTS4 encodes a nerolidol/linalool synthase. Transcripts of CuSTS3-1, CuSTS3-2 and CuSTS4 were abundant in young fruit at 60 days after flowering (DAF), flowers and leaves, respectively. Treatments with Xanthomonas citri subsp. citri (XCC), the causal agent of citrus canker and Penicillium italicum (PI), the cause of post-harvest fruit decay, and wounding up-regulated CuSTS3-1 in fruit and mainly CuSTS4 in leaves. Linalool, citral, geraniol and citronellol showed strong antibacterial and antifungal activities against XCC and PI in vitro, while most other mono-and sesquiterpenes, including limonene and gamma-terpinene, did not. Linalool, used at levels similar to those present in resistant Ponkan mandarin (Citrus reticulata Blanco) leaves, was able to inhibit growth of XCC in vitro. Compared to other five citrus.types, linalool accumulated at extraordinarily high levels in Ponkan mandarin leaves and was released at high amounts from their leaves, while it was hardly detectable in the most susceptible species, indicating that linalool biosynthesis and accumulation might be involved in plant defense against bacterial and fungal pathogens and be associated with field resistance to citrus canker. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
机译:分离了来自萨摩普通话(Citrus unshiu Marc。)的三个cDNA克隆,并在大肠杆菌中表达。 CuSTS3-1和CuSTS3-2编码芳樟醇合酶,而CuSTS4编码神经醇/芳樟醇合酶。开花后(DAF),花和叶分别在60天时,幼果中的CuSTS3-1,CuSTS3-2和CuSTS4的转录本丰富。柠檬黄单胞菌亚种的治疗。柑橘溃疡病和柑橘青霉(PI)的病原体柠檬(XCC),水果收获后腐烂的原因以及水果中CuSTS3-1的表达上调,主要是叶片中的CuSTS4的上调。芳樟醇,柠檬醛,香叶醇和香茅醇在体外对XCC和PI表现出强大的抗菌和抗真菌活性,而其他大多数单萜和倍半萜,包括柠檬烯和γ-萜品烯则没有。使用与抗性Ponkan普通话(Citrus reticulata Blanco)叶片中存在的含量相似的芳樟醇能够在体外抑制XCC的生长。与其他5种柑桔类型相比,芳樟醇在Ponkan普通话叶片中的积累非常高,并从其叶片中大量释放,而在最易感的物种中几乎无法检测到,表明芳樟醇的生物合成和积累可能与植物有关。对细菌和真菌病原体具有防御作用,并与柑橘溃疡病的田间抗性有关。 (C)2014 Elsevier Ireland Ltd.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号