...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant Science: An International Journal of Experimental Plant Biology >Differentially expressed genes in Populus simonii x Populus nigra in response to NaCl stress using cDNA-AFLP.
【24h】

Differentially expressed genes in Populus simonii x Populus nigra in response to NaCl stress using cDNA-AFLP.

机译：利用cDNA-AFLP响应NaCl胁迫而在小杨（Populus simonii）x黑杨（Populus nigra）中表达差异的基因。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Salinity is an important environmental factor limiting growth and productivity of plants, and affects almost every aspect of the plant physiology and biochemistry. The objective of this study was to apply cDNA-AFLP and to identify differentially expressed genes in response to NaCl stress vs. no-stress in Populus simonii x Populus nigra in order to develop genetic resources for genetic improvement. Selective amplification with 64 primer combinations allowed the visualization of 4407 transcript-derived fragments (TDFs), and 2027 were differentially expressed. Overall, 107 TDFs were re-sequenced successfully, and 86 unique sequences were identified in 10 functional categories based on their putative functions. A subset of these genes was selected for real-time PCR validation, which confirmed the differential expression patterns in the leaf tissues under NaCl stress vs. no stress. Differential expressed genes will be studied further for association with salt or drought-tolerance in P. simonii x P. nigra. This study suggests that cDNA-AFLP is a useful tool to serve as an initial step for characterizing transcriptional changes induced by NaCl salinity stress in P. simonii x P. nigra and provides resources for further study and application in genetic improvement and breeding. All unique sequences have been deposited in the Genbank as accession numbers GW672587-GW672672 for public use.
机译：盐度是限制植物生长和生产力的重要环境因素，几乎影响植物生理和生化的各个方面。这项研究的目的是应用cDNA-AFLP并识别西小杨x黑杨对NaCl胁迫和无胁迫的差异表达基因，以便开发遗传资源以进行遗传改良。用64个引物组合进行的选择性扩增可显示4407个转录本片段（TDF），并差异表达2027个。总体而言，成功地对107个TDF进行了重新测序，并根据其推定功能在10个功能类别中鉴定了86个独特序列。选择这些基因的一个子集进行实时PCR验证，这证实了NaCl胁迫与无胁迫下叶片组织中的差异表达模式。将进一步研究差异表达基因与P中的耐盐或耐旱性的关系。 simonii x P。老黑。这项研究表明，cDNA-AFLP是有用的工具，可用于表征NaCl盐胁迫下iP诱导的转录变化。 simonii x P。 nigra ，为进一步研究和在遗传改良和育种中的应用提供了资源。所有独特序列已作为登录号GW672587-GW672672保藏在Genbank中，以供公众使用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号