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首页> 外文期刊>Plant signaling & behavior >Extracellular hydrogen peroxide, produced through a respiratory burst oxidase/superoxide dismutase pathway, directs ingrowth wall formation in epidermal transfer cells of Vicia faba cotyledons.
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Extracellular hydrogen peroxide, produced through a respiratory burst oxidase/superoxide dismutase pathway, directs ingrowth wall formation in epidermal transfer cells of Vicia faba cotyledons.

机译:通过呼吸爆发氧化酶/超氧化物歧化酶途径产生的细胞外过氧化氢指导蚕豆子叶表皮转移细胞中向内生长壁的形成。

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摘要

The intricate, and often polarized, ingrowth walls of transfer cells (TCs) amplify their plasma membrane surface areas to confer a transport function of supporting high rates of nutrient exchange across apo-/symplasmic interfaces. The TC ingrowth wall comprises a uniform wall layer on which wall ingrowths are deposited. Signals and signal cascades inducing trans-differentiation events leading to formation of TC ingrowth walls are poorly understood. Vicia faba cotyledons offer a robust experimental model to examine TC induction as, when placed into culture, their adaxial epidermal cells rapidly (h) and synchronously form polarized ingrowth walls accessible for experimental observations. Using this model, we recently reported findings consistent with extracellular hydrogen peroxide, produced through a respiratory burst oxidase homolog/superoxide dismutase pathway, initiating cell wall biosynthetic activity and providing directional information guiding deposition of the polarized uniform wall. Our conclusions rested on observations derived from pharmacological manipulations of hydrogen peroxide production and correlative gene expression data sets. A series of additional studies were undertaken, the results of which verify that extracellular hydrogen peroxide contributes to regulating ingrowth wall formation and is generated by a respiratory burst oxidase homolog/superoxide dismutase pathway.
机译:转移细胞(TC)的复杂的且通常是极化的向内生长壁会放大其质膜表面积,从而赋予转运功能,以支持跨非营养/同质界面的高速率养分交换。 TC向内生长壁包括均匀的壁层,壁向内沉积在壁上。引起转分化事件并导致TC向内生长壁形成的信号和信号级联知之甚少。蚕豆子叶提供了一个强大的实验模型,可以检查TC诱导,因为将其放置在培养物中后,它们的表皮表皮细胞迅速(h)并同步形成极化的向内生长壁,便于实验观察。使用该模型,我们最近报道了与细胞外过氧化氢相一致的发现,这些过氧化氢通过呼吸爆发氧化酶同源物/超氧化物歧化酶途径产生,引发细胞壁的生物合成活性并提供指导极化均匀壁沉积的方向性信息。我们的结论基于过氧化氢产生的药理学操作和相关基因表达数据集的观察结果。进行了一系列其他研究,其结果证实细胞外过氧化氢有助于调节向内生长壁的形成,并由呼吸爆发氧化酶同系物/超氧化物歧化酶途径产生。

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