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首页> 外文期刊>Planta: An International Journal of Plant Biology >Cellular localization and levels of pectins and arabinogalactan proteins in olive (Olea europaea L.) pistil tissues during development: Implications for pollen-pistil interaction
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Cellular localization and levels of pectins and arabinogalactan proteins in olive (Olea europaea L.) pistil tissues during development: Implications for pollen-pistil interaction

机译：橄榄（Olea europaea L.）雌蕊组织在发育过程中的细胞定位以及果胶和阿拉伯半乳聚糖蛋白的水平：对花粉-雌蕊相互作用的影响

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摘要

Cell wall components in the pistil are involved in cell-cell recognition, nutrition and regulation of pollen tube growth. The aim of this work was to study the level, whole-organ distribution, and subcellular localization of pectins and arabinogalactan proteins (AGPs) in the olive developing pistil. Western blot analyses and immunolocalization with fluorescence and electron microscopy were carried out using a battery of antibodies recognizing different types of pectin epitopes (JIM7, JIM5, LM5, and LM6) and one anti-AGPs antibody (JIM13). In the olive pistil, highest levels of acid esterified and de-esterified pectins were observed at pollination. Moreover, pollination was accompanied by a slight decrease of the galactose-rich pectins pool, whereas arabinose-rich pectins were more abundant at that time. An increased expression of AGPs was also observed during pollination, in comparison to the pistil at the pre-anthesis stage. After pollination, the levels of pectins and AGPs declined significantly. Inmunofluorescence localization of pectins showed their different localization in the olive pistil. Pectins with galactose residues were located mainly in the cortical zones of the pistil, similar to the neutral pectins, which were found in the parenchyma and epidermis. In turn, the neutral pectins, which contain arabinose residues and AGPs, were localized predominantly in the stigmatic exudate, in the cell wall of secretory cells of the stigma, as well as in the transmitting tissue of the pistil during the pollination period. The differences in localization of pectins and AGPs are discussed in relation to their roles during olive pistil developmental course.
机译：雌蕊中的细胞壁成分参与细胞识别，营养和花粉管生长的调节。这项工作的目的是研究果胶和阿拉伯半乳聚糖蛋白（AGPs）在发育中的橄榄雌蕊中的水平，全器官分布和亚细胞定位。使用一系列识别不同类型果胶表位的抗体（JIM7，JIM5，LM5和LM6）和一种抗AGPs抗体（JIM13）​​，进行了蛋白质印迹分析和荧光和电子显微镜免疫定位。在橄榄雌蕊中，在授粉时观察到最高水平的酯化和去酯化果胶。此外，授粉过程中富含半乳糖的果胶池略有减少，而富含阿拉伯糖的果胶当时更为丰富。与花前期的雌蕊相比，在授粉过程中还观察到AGP的表达增加。授粉后，果胶和AGP的含量显着下降。果胶的免疫荧光定位显示它们在橄榄雌蕊中的定位不同。具有半乳糖残基的果胶主要位于雌蕊的皮质区域，类似于在薄壁组织和表皮中发现的中性果胶。继而，含有阿拉伯糖残基和AGP的中性果胶主要在授粉期间定位于柱头分泌物，柱头分泌细胞的细胞壁以及雌蕊的传递组织中。讨论了果胶和AGP在橄榄雌蕊发育过程中的作用方面的差异。

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