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首页> 外文期刊>Planta: An International Journal of Plant Biology >Heterologous expression of the BABY BOOM AP2/ERF transcription factor enhances the regeneration capacity of tobacco (Nicotiana tabacum L.)
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Heterologous expression of the BABY BOOM AP2/ERF transcription factor enhances the regeneration capacity of tobacco (Nicotiana tabacum L.)

机译：BABY BOOM AP2 / ERF转录因子的异源表达增强了烟草（Nicotiana tabacum L.）的再生能力

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摘要

Gain-of-function studies have shown that ectopic expression of the BABY BOOM (BBM) AP2/ERF domain transcription factor is sufficient to induce spontaneous somatic embryogenesis in Arabidopsis (Arabidopsis thaliana (L.) Heynh) and Brassica napus (B. napus L.) seedlings. Here we examined the effect of ectopic BBM expression on the development and regenerative capacity of tobacco (Nicotiana tabacum L.) through heterologous expression of Arabidopsis and B. napus BBM genes. 35S::BBM tobacco lines exhibited a number of the phenotypes previously observed in 35S::BBM Arabidopsis and B. napus transgenics, including callus formation, leaf rumpling, and sterility, but they did not undergo spontaneous somatic embryogenesis. 35S::BBM plants with severe ectopic expression phenotypes could not be assessed for enhanced regeneration at the seedling stage due to complete male and female sterility of the primary transformants, therefore fertile BBM ectopic expression lines with strong misexpression phenotypes were generated by expressing a steroid-inducible, post-translationally controlled BBM fusion protein (BBM:GR) under the control of a 35S promoter. These lines exhibited spontaneous shoot and root formation, while somatic embryogenesis could be induced from in-vitro germinated seedling hypocotyls cultured on media supplemented with cytokinin. Together these results suggest that ectopic BBM expression in transgenic tobacco also activates cell proliferation pathways, but differences exist between Arabidopsis/B. napus and N. tabacum with respect to their competence to respond to the BBM signalling molecule.
机译：功能获得的研究表明，BABY BOOM（BBM）AP2 / ERF结构域转录因子的异位表达足以诱导拟南芥（Arabidopsis thaliana（L.）Heynh）和甘蓝型油菜（B. napus L）的自发体细胞胚发生。 。）幼苗。在这里，我们通过异源表达拟南芥和油菜BBM基因，研究了异位BBM表达对烟草（Nicotiana tabacum L.）发育和再生能力的影响。 35S :: BBM烟草系表现出许多以前在35S :: BBM拟南芥和甘蓝型芽孢杆菌转基因中观察到的表型，包括愈伤组织形成，叶片起皱和不育，但它们并未经历自发的体细胞胚发生。由于主要转化子的雄性和雌性完全不育，无法评估具有严重异位表达表型的35S :: BBM植物在苗期的再生增强，因此，通过表达类固醇可以生成具有强错表达表型的可育BBM异位表达系诱导型，翻译后控制的BBM融合蛋白（BBM：GR）在35S启动子的控制下。这些系表现出自发的芽和根形成，而体细胞胚发生可以通过在补充细胞分裂素的培养基上培养的体外发芽的幼苗下胚轴来诱导。这些结果共同表明，转基因烟草中异位BBM的表达也激活细胞增殖途径，但拟南芥/ B之间存在差异。的油菜和烟草对它们对BBM信号分子的反应能力。

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