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Effects of growth and measurement light intensities on temperature dependence of CO assimilation rate in tobacco leaves

机译:生长和测量光强度对烟叶CO同化率的温度依赖性的影响

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Effects of growth light intensity on the temperature dependence of CO assimilation rate were studied in tobacco (Nicotiana tabacum) because growth light intensity alters nitrogen allocation between photosynthetic components. Leaf nitrogen, ribulose 1p"5-bisphosphate carboxylase/oxygenase (Rubisco) and cytochrome f (cyt f) contents increased with increasing growth light intensity, but the cyt f/Rubisco ratio was unaltered. Mesophyll conductance to CO diffusion (gm) measured with carbon isotope discrimination increased with growth light intensity but not with measuring light intensity. The responses of CO assimilation rate to chloroplast CO concentration (Cc) at different light intensities and temperatures were used to estimate the maximum carboxylation rate of Rubisco (Vcmax) and the chloroplast electron transport rate (J). Maximum electron transport rates were linearly related to cyt f content at any given temperature (e.g. 115 and 179 omol electrons molp# cyt f sp# at 25 and 40 pC, respectively). The chloroplast CO concentration (Ctrans) at which the transition from RuBP carboxylation to RuBP regeneration limitation occurred increased with leaf temperature and was independent of growth light intensity, consistent with the constant ratio of cyt f/Rubisco. In tobacco, CO assimilation rate at 380 omol molp# CO concentration and high light was limited by RuBP carboxylation above 32 pC and by RuBP regeneration below 32 pC.
机译:研究了生长光强度对烟草(Nicotiana tabacum)中CO同化率的温度依赖性的影响,因为生长光强度改变了光合成分之间的氮分配。叶片氮,核糖1p“ 5-二磷酸羧化酶/加氧酶(Rubisco)和细胞色素f(cyt f)的含量随生长光强度的增加而增加,但cyt f / Rubisco的比例未改变。碳同位素分辨力随生长光强度的增加而增加,但不随光强度的增加而增加,使用CO同化率对叶绿体的响应通过不同光强度和温度下CO浓度(Cc)的估算来估算Rubisco和叶绿体的最大羧化率电子传输速率(J)。在任何给定温度下,最大电子传输速率与cyt f含量线性相关(例如在25和40 pC时分别为115和179 omol电子molp#cyt f sp#)。 ),从RuBP羧化到RuBP再生限制的转变随叶片温度的升高而增加,并且与生长光强度无关与cyt f / Rubisco的恒定比率保持一致。在烟草中,在高于32 pC的RuBP羧化和低于32 pC的RuBP再生限制了380 omol molp#CO浓度和高光下的CO同化率。

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