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首页> 外文期刊>Plant, Cell & Environment >Dual role for 14-3-3 proteins and ABF transcription factors in gibberellic acid and abscisic acid signalling in barley (Hordeum vulgare) aleurone cells
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Dual role for 14-3-3 proteins and ABF transcription factors in gibberellic acid and abscisic acid signalling in barley (Hordeum vulgare) aleurone cells

机译：大麦（Hordeum vulgare）糊粉细胞中14-3-3蛋白和ABF转录因子在赤霉素和脱落酸信号传导中的双重作用

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The balance of gibberellins [gibberellic acid (GA)] and abscisic acid (ABA) is a determining factor during transition of embryogenesis and seed germination. Recently, we showed that 14-3-3 proteins are important in ABA signalling in barley aleurone cells. Using 14-3-3 RNAi constructs in the barley aleurone transient expression system, we demonstrate here that silencing of each 14-3-3 isoform suppresses GA induction of the alpha-amylase gene. 14-3-3 Proteins interact with ABA-responsive element (ABRE) binding factors HvABF1, 2 and 3, and here we show that these transcription factors also interact with the ABA-responsive kinase PKABA1, a kinase that mediates cross-talk between the GA and ABA pathway. ABF1 and ABF2 have a function in both signalling pathways as: (1) ectopic expression of wild-type ABF1 and mutant ABF2, lacking the 14-3-3 interaction domain, transactivates the ABA inducible HVA1 gene; and (2) GA induction of the alpha-amylase gene is repressed by ectopic expression of wild-type ABF1 and 2. Mutant ABF1 and 2 were still effective repressors of GA signalling. In summary, our data provide evidence that 14-3-3 proteins and members of the ABF transcription factor family have a regulatory function in the GA pathway and suggest that PKABA1 and ABF transcription factors are cross-talk intermediates in ABA and GA signalling.
机译：赤霉素[赤霉素（GA）]和脱落酸（ABA）的平衡是胚胎发生和种子萌发过渡期间的决定因素。最近，我们显示14-3-3蛋白在大麦糊粉细胞的ABA信号传导中很重要。在大麦糊粉瞬时表达系统中使用14-3-3 RNAi构建体，我们在这里证明每个14-3-3同工型的沉默都可以抑制GA诱导的α-淀粉酶基因。 14-3-3蛋白与ABA响应元件（ABRE）结合因子HvABF1、2和3相互作用，在这里我们显示这些转录因子还与ABA响应激酶PKABA1相互作用，该激酶介导了蛋白之间的串扰。 GA和ABA途径。 ABF1和ABF2在两种信号通路中均具有以下功能：（1）异位表达野生型ABF1和突变型ABF2，缺少14-3-3相互作用域，可激活ABA诱导型HVA1基因。 （2）野生型ABF1和2的异位表达抑制了α-淀粉酶基因的GA诱导。突变的ABF1和2仍然是GA信号转导的有效阻遏物。总而言之，我们的数据提供了证据，表明14-3-3蛋白和ABF转录因子家族的成员在GA通路中具有调节功能，并表明PKABA1和ABF转录因子是ABA和GA信号传导的串扰中间物。

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