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首页> 外文期刊>Biomaterials >Improving arterial prosthesis neo-endothelialization: Application of a proactive VEGF construct onto PTFE surfaces
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Improving arterial prosthesis neo-endothelialization: Application of a proactive VEGF construct onto PTFE surfaces

机译：改善动脉假体的新内皮功能：将主动性VEGF构建体应用于PTFE表面

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The formation of a confluent endothelium on expanded polytetrafluoroethylene (PTFE) vascular prostheses has never been observed. This lack of endothelialization is known to be one of the main reasons leading to the development of thromboses and/or intimal hyperplasia. In this context, several efforts were put forward to promote endothelial cell coverage on the internal surface of synthetic vascular prostheses. The goal of the present study was to immobilize the vascular endothelial growth factor (VEGF) onto Teflon (R) PTFE surfaces to generate a proactive polymer construct favoring interaction with endothelial cells. An ammonia plasma treatment was first used to graft amino groups on PTFE films. Subsequent reactions were performed to covalently bind human serum albumin (HSA) on the polymer surface and to load this protein with negative charges, which allows adsorbtion of VEGF onto HSA via strong electrostatic interactions. X-ray photoelectron spectroscopy (XPS) experiments along with surface derivatization strategies were performed between each synthesis step to ascertain the occurrence of the various molecules surface immobilization. Finally, the electrostatic binding of VEGF to the negatively charged HSA matrix was performed and validated by ELISA. Endothelial cell adhesion and migration experiments were carried out to validate the potential of this VEGF-containing biological construct to act as a proactive media toward the development of endothelial cells. (c) 2005 Elsevier Ltd. All rights reserved.
机译：从未观察到在膨胀的聚四氟乙烯（PTFE）血管假体上形成融合内皮。已知缺乏内皮化是导致血栓形成和/或内膜增生的主要原因之一。在这种情况下，人们提出了一些努力来促进合成血管假体的内表面上的内皮细胞覆盖。本研究的目的是将血管内皮生长因子（VEGF）固定在特氟隆（R）PTFE表面上，以产生有利于与内皮细胞相互作用的主动聚合物构建体。首先使用氨等离子体处理将PTFE膜上的氨基接枝。进行随后的反应以在聚合物表面上共价结合人血清白蛋白（HSA），并使该蛋白带有负电荷，从而允许VEGF通过强静电相互作用吸附到HSA上。在每个合成步骤之间进行X射线光电子能谱（XPS）实验以及表面衍生化策略，以确定各种分子表面固定化的发生。最后，进行VEGF与带负电荷的HSA基质的静电结合，并通过ELISA进行验证。进行了内皮细胞粘附和迁移实验，以验证这种含VEGF的生物构建体作为促进内皮细胞发育的主动介质的潜力。 （c）2005 Elsevier Ltd.保留所有权利。

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