...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant Molecular Biology >Differential expression of two cinnamate 4-hydroxylase genes in 'Valencia'orange (Citrus sinensis Osbeck)
【24h】

Differential expression of two cinnamate 4-hydroxylase genes in 'Valencia'orange (Citrus sinensis Osbeck)

机译：两个肉桂酸4-羟化酶基因在'Valencia'orange（Citrus sinensis Osbeck）中的差异表达

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Two different full-length cDNAs for cinnamate 4-hydroxylase (C4H1 and C4H2) were isolated from Citrus sinensis Osbeck cv. Valencia libraries. C4H1 (1708 bp) and C4H2 (1871 bp) share only 65% identity on nucleotide and 66% identity on the amino acid level, respectively. C4H1 is most homologous to a cinnamate 4-hydroxylase sequence from French bean (Phaseolus vulgaris), but codes for a unique N-terminus. C4H2 shows highest similarity to a poplar (Populus kitakamiensis) sequence, but also shows a unique N-terminus. The two genes are expressed differentially in orange flavedo, C4H2 is constitutive, C4H1 is wound-induced. In competitive RT-PCR, the mRNA for both genes in wounded and untreated tissue was quantified. C4H1 is strongly wound-inducible from `not detectable' to about 35 fg mRNA per 50 ng total RNA 8 h after wounding. The first detectable C4H1 mRNA was found 4 h after wounding. After reaching peak levels 4 h later the levels slightly declined, but stayed elevated until the end of the experiment (48 h). C4H2 is expressed 3-10 times higher than wound-induced C4H1 even in the control sample; wounding transiently increases the level of expression another 2-3 times. The existence of different N-termini and their effects on the possible role of both genes in phenylpropanoid pathways is discussed.
机译：肉桂酸4-羟化酶的两个不同的全长cDNA（C4H1和C4H2）是从柑桔Osbeck cv中分离出来的。巴伦西亚图书馆。 C4H1（1708 bp）和C4H2（1871 bp）在核苷酸水平上分别仅具有65％的同一性，在氨基酸水平上具有66％的同一性。 C4H1与来自菜豆（菜豆）的肉桂酸4-羟化酶序列最同源，但编码一个唯一的N端。 C4H2与白杨（Populus kitakamiensis）序列显示出最高的相似性，但也显示出唯一的N端。这两个基因在橙色的flavedo中差异表达，C4H2是组成型，C4H1是伤口诱导的。在竞争性RT-PCR中，对受伤和未治疗组织中两个基因的mRNA进行了定量。 C4H1可以在8小时后从“无法检测”强烈诱导为每50 ng总RNA约35 fg mRNA。受伤4小时后发现第一个可检测的C4H1 mRNA。在达到峰值水平4小时后，其水平略有下降，但一直保持升高直到实验结束（48小时）。即使在对照样品中，C4H2的表达也比伤口诱导的C4H1高3-10倍;伤口暂时又增加了2-3倍的表达水平。讨论了不同的N末端的存在及其对两个基因在苯丙烷途径中可能作用的影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号