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首页> 外文期刊>Chemical Senses >Putative Odorant-degrading Esterase cDNA from the Moth Mamestra brassicae:Cloning and Expression Patterns in Male and Female Antennae
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Putative Odorant-degrading Esterase cDNA from the Moth Mamestra brassicae:Cloning and Expression Patterns in Male and Female Antennae

机译：蝴蝶蛾的臭味降解酯酶cDNA的假定：雄性和雌性天线的克隆和表达模式

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摘要

An esterase cDNA was isolated from the cabbage armyworm Mamestra brassicae antennae by PCR strategy.The full-length cDNA,designated as Mbra-EST,contains a 1638 bp open reading frame encoding a predicted protein of 546 amino acids.This predicted protein presents the structural characteristics of known insect carboxyl-esterases,in particular the Ser-His-Glu catalytic triad.The expression pattern of the gene was studied by RT-PCR,Northern-blot and in situ hybridization.The ribosomal protein rpL8 gene from M.brassicae was also cloned to obtain a normalized tool for the comparative gene expression studies.Mbra-EST transcripts are specifically expressed in the antennae of males and females and in the proboscis of males.In antennae of both sexes,expression is restricted to the olfactory sensilla trichodea,suggesting a role in degradation of odorant acetate compounds,such as pheromones as well as plant volatile acetate components.
机译：通过PCR策略从甘蓝夜蛾幼虫触角分离出酯酶cDNA。全长cDNA，称为Mbra-EST，包含一个1638 bp的开放阅读框，编码546个氨基酸的预测蛋白。通过RT-PCR，Northern-blot和原位杂交研究了该基因的表达模式。研究了芥蓝的核糖体蛋白rpL8基因。还克隆了该克隆，以获得用于比较基因表达研究的归一化工具。Mbra-EST转录本在雄性和雌性触角以及雄性长舌中都有特异性表达。在两性触角中，表达仅限于嗅觉性毛细线虫，提示在降解有气味的乙酸酯化合物（如信息素）以及植物挥发性乙酸酯成分中起一定作用。

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