...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant Pathology >First report of Chrysanthemum stem necrosis virus (CSNV) in the UK
【24h】

First report of Chrysanthemum stem necrosis virus (CSNV) in the UK

机译：英国首次报告菊花干坏死病毒（CSNV）

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In November 2002, samples were received from an all-year-round chrysanthemum crop growing under glass in south-west England. The crop consisted of cvs Fiji and Calabria, cuttings of which originated from Brazil. The samples had distinct dark stem lesions and some leaf necrosis. Given these symptoms and the absence of any fungal pathogens, the plants were tested by ELISA for the presence of three tospoviruses Chrysanthemum stem necrosis virus (CSNV) was detected using a polyclonal DAS-ELISA kit (DSMZ, Braunschweig, Germany), Neither Tomato spotted wilt virus nor Impatient necrotic spot virus were detected When examined electron-microscopically, sap from the samples was found to contain spherical tospovirus-like vims particles of 90-110 nm in diameter. Mechanical inoculation of sap from affected stem material to a range of indicator plant species resulted in symptoms typical of CSNV (Bezerra et al, 1999), including systemic mosaic in Nicotiana benthamiana; these indicator plants showing symptoms tested positive for CSNV when tested by ELISA. RT-PCR using degenerate tospovirus primers BR60 and BR65 (Eiras et al., 2001) was also performed on extracts taken from chrysanthemum stem tissue showing symptoms and a single productof the correct predicted size (453 bp) was obtained; this product was sequenced and shown to share 98% identity with the 5' end of the capsid protein gene of a published CSNV sequence (AF067068). The infected crop has now been removed and measures taken to eradicate the infection. As CSNV has previously been reported only from Brazil and the Netherlands (Bezerra et al, 1999; Verhoeven et al., 1996), this is the first report of its occurrence in the UK.
机译：在2002年11月，从英格兰西南部玻璃下生长的常年菊花作物中获取了样品。作物包括斐济和卡拉布里亚cvs，其中插穗来自巴西。样品具有明显的深色茎损伤和一些叶片坏死。考虑到这些症状并且没有任何真菌病原体，使用多克隆DAS-ELISA试剂盒（DSMZ，德国不伦瑞克，德国），通过ELISA对植物进行了三株弓形病毒的检测，检测到了菊花干坏死病毒（CSNV）。检测到枯萎病毒或急躁坏死斑点病毒。用电子显微镜检查时，发现样品中的汁液含有直径为90-110 nm的球形痘病毒样病毒颗粒。机械地将受影响的树干材料中的汁液机械接种到一系列指示性植物物种中，导致典型的CSNV症状（Bezerra等，1999），包括本氏烟草的系统性镶嵌;这些通过ELISA测试显示出对CSNV呈阳性的症状的指示植物。还使用简并的​​脊灰病毒引物BR60和BR65（Eiras等，2001）对显示症状的菊花茎组织提取物进行了RT-PCR，获得了具有正确预测大小（453 bp）的单一产物;对该产物进行了测序，显示与已发布的CSNV序列（AF067068）的衣壳蛋白基因的5'末端具有98％的同一性。现在已去除了受感染的农作物，并采取了消除感染的措施。由于以前仅从巴西和荷兰报告了CSNV（Bezerra等人，1999; Verhoeven等人，1996），这是其在英国的首次报道。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号