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Relative importance of seed-tuber and soilborne inoculum in causing black dot disease of potato

机译:块茎和土壤传播接种物在引起马铃薯黑点病中的相对重要性

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Controlled-environment and field experiments were done to quantify the individual contribution of seed-tuber and soilborne inoculum of Colletotrichum coccodes in causing black dot disease of potato tubers. Seed-tuber and soilborne inocula of C. coccodes were quantified using an existing real-time PCR assay and related to subsequent incidence and severity of disease. In four field trials, a controlled-environment experiment and through the monitoring of 122 commercial crops, seed-tuber inoculum was found to be relatively less important than soilborne inoculum in causing black dot, and the level of seed-tuber inoculum did not significantly affect either the incidence or severity of disease or the percentage of progeny tubers deemed unmarketable. By contrast, soilborne inoculum had the potential to result in high levels of disease and the level of C. coccodes soil infestation (pg DNA g~(-1) soil) was found to have a significant effect. At soil infestation levels below 100 pg DNA C. coccodes g~(-1) soil, 7% of commercial crops had an incidence of black dot greater than 20%, increasing to 40% and 57% of crops at levels of 100-1000 pg g~(-1) and >1000 pg g~(-1) soil, respectively. These arbitrary threshold levels for soilborne inoculum related to disease risk are discussed. Interpretation of disease risk based on inoculum levels must, in the future, be informed by agronomic variables and potential control strategies.
机译:进行了对照环境和田间试验,以量化种子块茎和炭疽菌的土壤传播接种物在引起马铃薯块茎黑点病中的作用。使用现有的实时PCR测定法对球囊念珠菌的种子块茎和土壤传播接种进行了定量,并与随后的疾病发病率和严重程度相关。在四项田间试验,一项受控环境实验中,并通过监测122种商品作物,发现块茎接种物在引起黑点方面比土壤传播接种物的重要性相对要低,并且块茎接种物的水平并未显着影响疾病的发生率或严重性,或认为无法销售的后代块茎的百分比。相比之下,土壤传播的接种物有可能导致高水平的疾病,并且发现C. coccodes土壤侵染水平(pg DNA g〜(-1)土壤)具有显着影响。在低于100 pg DNA C.coccodes g〜(-1)土壤的土壤侵染水平下,有7%的商业作物黑点发生率大于20%,在100-1000的水平下增加到40%和57% pg g〜(-1)和> 1000 pg g〜(-1)土壤。讨论了与疾病风险有关的土壤传播接种物的这些任意阈值水平。将来,必须通过农艺变量和潜在的控制策略来解释基于接种量的疾病风险。

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