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Genetic diversity in the mango malformation pathogen and development of a PCR assay

机译：芒果畸形病原体的遗传多样性和PCR检测方法的发展

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摘要

Malformation is a destructive disease of mango, Mangifera indica. Its causal agent possesses the morphological features of Fusarium subglutinans, a species whose taxonomy and nomenclature has recently been in a state of flux. Genetic diversity was examined among 74 F. subglutinans-like isolates from malformed mango in Brazil, Egypt, Florida (USA), India, Israel and South Africa. With nitrate-nonutilizing (nit) autotrophic mutants, seven vegatative compatibility groups (VCGs) were identified. Three of the VCGs were found in a single country, and VCG diversity was greatest in Egypt and the USA where, respectively, four and three different VCGs were found. RAPD profiles generated with greatest in Egypt and primers were variable among isolates in different VCGs, but were generally uniform for isolates within a VCG. In PCR assays, a 20-mer primer pair that was developed previously to identify F.subglutinanas from maize (mating population [MP]-E of the Gibberella fujikuroi complex) also amplified a specific 448 bp fragment for isolates of F.sacchari from sugarcane (MP-B) and what was probably F.circinatum (pine, MP-H), With the exception of three isolates from Brazil, it did not amplify the fragment from E.subglutinans-like isolates from mango. A second pair of 20-mer primers was developed from a unique fragment in the RAPD assays. It amplified a specific 608 bp fragment for 51 of 54 isolates from mango (all but the three Brazilian isolates). It also amplified a smaller, 550 bp fragment from isolates of F.nygamai (MP-G), but did not amplify DNA of isolates of any other taxon of Fusarium that was tested.
机译：畸形是芒果Mangifera的破坏性疾病。其病原体具有枯萎镰刀菌的形态特征，该物种的分类学和命名法最近处于变动状态。在巴西，埃及，佛罗里达（美国），印度，以色列和南非的74个芒果形畸形胶质瘤分离株中检测了遗传多样性。与硝酸盐不利用（nit）自养突变体，确定了七个素食相容性组（VCGs）。在单个国家中发现了三个VCG，在埃及和美国分别发现了四个和三个不同的VCG，VCG多样性最大。在埃及和引物中产生最大的RAPD图谱在不同VCG中的分离株之间是可变的，但对于VCG内的分离株通常是一致的。在PCR分析中，先前开发的一种20-mer引物对可从玉米中鉴定出谷氨酸镰刀菌（Fujikuroi复合体的交配种群[MP] -E），还扩增了一个448 bp的特异性片段，用于分离甘蔗糖酵母。 （MP-B）和可能的圆环拟南芥（pine，MP-H），除了来自巴西的三种分离株外，它均未扩增来自芒果的类似大肠杆菌的分离物。从RAPD分析中的独特片段开发出第二对20-mer引物。它为来自芒果的54个分离株中的51个分离了一个特定的608 bp片段（除了三个巴西分离株）。它也从F.nygamai（MP-G）分离株中扩增了一个较小的550 bp片段，但没有扩增经测试的镰刀菌其他任何分类群的分离株的DNA。

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