Development of Recombinant antibody Elisa test for the detection of Polymyxa betae and its use in resistance screening

C.S.Kingsnorth; M.J.C.Asher; D.M.Chwarszczynska; M.C.Luterbacher; E.S.Mutasa-Gottgens; G.J.P.Keane

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Development of Recombinant antibody Elisa test for the detection of Polymyxa betae and its use in resistance screening

机译：用于检测多粘菌βe的重组抗体Elisa试验的开发及其在抗性筛选中的应用

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An ELISA test was developed for the quantitative detectin of the obligate parasite Polymyxa betae,the vector of Beet necrotic yellow vein virus (BNYVV),in infected sugarbeet roots.The test used monoclonal and polyclonal antiobodies raised to a recombinantly expressed glutathione-S-transferase (GST)from P.betae.A close correlation was found between the number of P.betaz zoospores in serially diluted suspensions and absorbance values in the ELISA test.Timecourse studies of plants grown in naturally infested solis in controlled environment tests demonstrated the value of the ELISA test in screening for P.betae resistance.In preliminary tests,P.betae-resistant accessions of the wild sea beet (Beta vulgaris ssp.maritima),which might be used to restrict the transmission of BNYVV,were identified.

机译：针对被感染的甜菜根中甜菜坏死性黄脉病毒（BNYVV）的载体，开发了一种ELISA检测方法，用于定量检测甜菜坏死黄脉病毒（BNYVV）的专性寄生虫Polymyxa betae，该测试使用了单克隆抗体和多克隆抗体，产生了重组表达的谷胱甘肽S转移酶。（PST）（GST）在连续稀释的悬浮液中发现的P.betaz游动孢子数量与ELISA测试中的吸光度值密切相关。在受控环境测试中，自然侵染的土壤中生长的植物的时程研究表明，在初步测试中，鉴定了可用于限制BNYVV传播的野生甜菜（Beta vulgaris ssp.maritima）的P.betae抗性种质。

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来源
《Plant Pathology》 |2003年第6期|共8页
作者
C.S.Kingsnorth; M.J.C.Asher; D.M.Chwarszczynska; M.C.Luterbacher; E.S.Mutasa-Gottgens; G.J.P.Keane;
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正文语种 eng
中图分类植物保护;
关键词
ELISA; glutathione-S-transferase; His.-tag fusion protein; Polymyxa Betae; rhizomania; sugarbeet;

机译：酶联免疫吸附;谷胱甘肽-S-转移酶;组氨酸标签融合蛋白;多粘菌;根瘤菌;糖用甜菜;

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1. Development of a recombinant antibody ELISA test for the detection of Polymyxa betae and its use in resistance screening [J] . Kingsnorth CS, Asher MJC, Keane GJP, Plant Pathology . 2003,第6期

机译：用于检测多粘菌素的重组抗体ELISA试验的开发及其在抗性筛选中的应用
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4. DEVELOPMENT OF INSECT-LARVAE DERIVED ELISA TESTS FOR THE DETECTION OF ANTIBODIES TO REPLICASE AND CAPSID PROTEINS OF PORCINE CIRCOVIRUS TYPE 2 [C] . E. Perez-Martin, L. Grau-Roma, JM. Argilaguet, Congress ofthe International Pig Veterinary Society . 2008

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6. Sensitive and specific detection of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)—Specific IgM and IgG antibodies in human sera using recombinant CCHFV nucleoprotein as antigen in μ-capture and IgG immune complex (IC) ELISA tests [O] . Petra Emmerich, Angela Mika, Ronald von Possel, 2018

机译：克里米亚-刚果出血热病毒（CCHFV）的灵敏和特异性检测-使用重组CCHFV核蛋白作为抗原的μ捕获和IgG免疫复合物（IC）ELISA试验检测人血清中的特定IgM和IgG抗体
7. Development of a recombinant antibody ELISA test for the detection of Polymyxa betae and its use in resistance screening [O] . Kingsnorth, C.S., Asher, M. J. C., Keane, G.J.P., 2003

机译：开发用于多粘菌betae的检测的重组抗体ELIsa试验及其在抗性筛选中的用途
8. Indirect Immunoglobulin G (IgG) and IgM Enzyme-Linked Immunosorbent Assays (ELISAs) and IgM Capture ELISA for Detection of Antibodies to Lipopolysaccharide in Adult Typhoid Fever Patients in Pakistan. [R] . Sippel, J., Bukhtiari, N., Awan, M. B., 1989

机译：间接免疫球蛋白G（IgG）和Igm酶联免疫吸附试验（ELIsas）和Igm捕获ELIsa检测巴基斯坦成年伤寒患者脂多糖抗体。

Development of Recombinant antibody Elisa test for the detection of Polymyxa betae and its use in resistance screening

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