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首页> 外文期刊>Plant Pathology >Analysis of Iris yellow spot virus replication in vector and non-vector thrips species
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Analysis of Iris yellow spot virus replication in vector and non-vector thrips species

机译：鸢尾黄斑病毒在媒介和非媒介蓟马种类中的复制分析

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摘要

Iris yellow spot virus (IYSV, genus Tospovirus) is a viral disease of bulb and seed onion crops and is transmitted by Thrips tabaci. Foliage damage of up to 75% has been reported in Kenya and Uganda. In this study, the rate of IYSV replication in the larva, pupa and adult stages of T. tabaci and other non-vector thrips species and colour forms such as Frankliniella occidentalis, F. scbultzei (dark) and F. schultzei (pale) was evaluated by monitoring relative levels of nucleocapsid (N) and non-structural (NSs) proteins using N- and NSs-specific antibodies. The effect of IYSV replication on mortality of thrips was also determined. N protein levels increased in all three stages of lYSV-fed T. tabaci, indicating replication of IYSV. In IYSV-fed non-vector thrips, the increase of N protein levels in the larval stage was lower than IYSV-fed T. tabaci but higher than their healthy counterparts. The N protein levels did not increase at pupal and adult stages. NSs protein was not detected in first instar of either vector or non-vector thrips species. After a 4 h post-acquisition period, a significant increase in NSs proteins was only observed in IYSV-fed T. tabaci, clearly differentiating vectors and non-vectors of IYSV. IYSV replication did not influence the survival of the vector thrips species, T. tabaci populations or the non-vector thrips species. This study indicates the effectiveness of monitoring non-structural proteins such as NSs, compared to nucleocapsid proteins, for differentiating vectors and non-vectors of IYSV.
机译：虹膜黄斑病毒（IYSV，弓形病毒属）是鳞茎和种洋葱作物的病毒性疾病，由烟蓟马传播。在肯尼亚和乌干达，据报导，高达75％的叶子受损。在这项研究中，IYSV在烟粉虱和其他非媒介蓟马种类和颜色形式（如富兰克氏菌，烟粉虱（暗）和schultzei（苍白））的幼虫，和成虫阶段的复制速率为通过使用N和NSs特异性抗体监测核衣壳（N）和非结构（NSs）蛋白的相对水平进行评估。还确定了IYSV复制对蓟马死亡率的影响。在由YYSV饲喂的烟粉虱的所有三个阶段中，N蛋白水平均升高，表明IYSV复制。在IYSV喂养的非载体蓟马中，幼虫阶段N蛋白水平的增加低于IYSV喂养的烟粉虱，但高于其健康同龄人。在protein和成年阶段，N蛋白水平没有增加。在载体或非载体蓟马物种的第一龄中均未检测到NSs蛋白。采集后4小时后，仅在IYSV饲喂的烟粉虱中明显观察到NSs蛋白的明显增加，从而明显区分了IYSV的载体和非载体。 IYSV复制不影响载体蓟马种类，烟粉虱种群或非载体蓟马种类的存活。这项研究表明，与核衣壳蛋白相比，监测非结构蛋白（例如NSs）对于区分IYSV载体和非载体的有效性。

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