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Population structure of Sclerotinia sclerotiorum in crop and wild hosts in the UK

机译:英国农作物和野生寄主中核盘菌的种群结构

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Sclerotinia sclerotiorum is an important pathogen of many crop plants which also infects wild hosts. The population structure of this fungus was studied for different crop plants and Ranunculus acris (meadow buttercup) in the UK using eight microsatellite markers and sequenced sections of the intergenic spacer (IGS) region of the rRNA gene and the elongation factor 1-alpha (EF) gene. A total of 228 microsatellite haplotypes were identified within 384 isolates from 12 S. sclerotiorum populations sampled in England and Wales. One microsatellite haplotype was generally found at high frequency in each population and was distributed widely across different hosts, locations and years. Fourteen IGS and five EF haplotypes were found in the 12 populations, with six IGS haplotypes and one EF haplotype exclusive to buttercup. Analysis of published sequences for S. sclerotiorum populations from the USA, Canada, New Zealand and Norway showed that three of the IGS haplotypes and one EF haplotype were widely distributed, while eight IGS haplotypes were only found in the UK. Although common microsatellite and IGS/EF haplotypes were found on different hosts in the UK, there was evidence of differentiation, particularly for one isolated population on buttercup. However, overall there was no consistent differentiation of S. sclerotiorum populations from buttercup and crop hosts. Sclerotinia sclerotiorum therefore has a multiclonal population structure in the UK and the wide distribution of one microsatellite haplotype suggests spatial mixing at a national scale. The related species S. subarctica was also identified in one buttercup population.
机译:核盘菌核盘菌是许多农作物的重要病原体,也感染野生宿主。使用8个微卫星标记以及rRNA基因的基因间隔区(IGS)区域和延伸因子1-alpha(EF)的序列切片,研究了该真菌在英国不同农作物和毛an(草甸毛cup)的种群结构。 )基因。在来自英格兰和威尔士的12个菌核菌的384种分离物中,共鉴定出228种微卫星单倍型。通常在每个人群中以高频率发现一种微卫星单倍型,并广泛分布于不同的宿主,位置和年份。在12个种群中发现了14个IGS和5个EF单倍型,其中有6个IGS单倍型和1个EF单倍型仅属于毛cup。对来自美国,加拿大,新西兰和挪威的核盘菌菌群种群已发表序列的分析表明,广泛分布了三种IGS单倍型和一种EF单倍型,而仅在英国发现了八种IGS单倍型。尽管在英国的不同宿主上发现了常见的微卫星和IGS / EF单倍型,但有证据表明存在分化,特别是对于一个孤立的毛butter种群。但是,总体上,菌核链球菌种群与毛cup和农作物寄主之间没有一致的区别。因此,核盘菌核盘菌在英国具有多克隆种群结构,一种微卫星单倍型的广泛分布表明全国范围内的空间混合。在一个毛cup种群中也鉴定到了近缘种S. subarctica。

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