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首页> 外文期刊>Plant Pathology >GENETIC COMPARISON OF THE PEACH YELLOW LEAF ROLL AGENT WITH EUROPEAN FRUIT TREE PHYTOPLASMAS OF THE APPLE PROLIFERATION GROUP
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GENETIC COMPARISON OF THE PEACH YELLOW LEAF ROLL AGENT WITH EUROPEAN FRUIT TREE PHYTOPLASMAS OF THE APPLE PROLIFERATION GROUP

机译：苹果繁殖组桃黄色叶生叶代理与欧洲果树植物遗传比较

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摘要

Restriction fragment length polymorphism (RFLP) analysis of PCR-amplified ribosomal DNA and Southern blot hybridization using cloned chromosomal DNA fragments from the apple proliferation (AP) phytoplasma as probes were used to investigate the genetic relationship of the California peach yellow leaf roll (PYLR) agent with phytoplasmas causing fruit tree diseases in Europe. This comparison showed that the California PYLR phytoplasma is closely related to apple proliferation (AP), pear decline, and European stone fruit yellows phytoplasmas and that it is a member of the phylogenetic AP group. The PYLR agent could clearly be distinguished from the AP and European stone fruit yellows phytoplasmas by Southern blot hybridization with DNA fragments from the AP phytoplasma and by RFLP analysis of ribosomal DNA employing SspI, BsaAI, and RsaI restriction endonucleases. However, the PYLR phytoplasma was indistinguishable from the pear decline agent by RFLP analysis of PCR-amplified ribosomal DNA.
机译：PCR扩增的核糖体DNA的限制性片段长度多态性（RFLP）分析和Southern blot杂交，使用来自苹果增殖（AP）植物质体的克隆染色体DNA片段作为探针，研究加利福尼亚桃黄卷叶（PYLR）的遗传关系在欧洲引起植物枯萎病的果蝇病原体。该比较表明，加利福尼亚州的PYLR植物质体与苹果增生（AP），梨减少和欧洲核果黄色植物质体密切相关，并且它是系统发育AP组的成员。通过与AP植物质体的DNA片段进行Southern印迹杂交，以及使用SspI，BsaAI和RsaI限制性核酸内切酶对核糖体DNA进行RFLP分析，可以将PYLR试剂与AP和欧洲核果黄植物质体明显区分开。然而，通过PCR扩增的核糖体DNA的RFLP分析，PYLR植物质与梨减低剂没有区别。

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