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首页> 外文期刊>Plant Pathology >Erwinia pyrifoliae, an Erwinia species different from Erwinia amylovora,causes a necrotic disease of Asian pear trees
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Erwinia pyrifoliae, an Erwinia species different from Erwinia amylovora,causes a necrotic disease of Asian pear trees

机译：欧文氏桐（Erwinia pyrifoliae）是一种不同于欧文氏木霉的欧文氏菌，引起亚洲梨树的坏死病

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摘要

Bacteria from necrotic branches of Asian pear trees (Pyrus pyrifolia) in Korea were consistently isolated as white colonies on nutrient agar and formed mucoid, slightly yellow colonies on a minimal medium with topper sulphate. Isolates with this colony morphology were studied in a series of microbiological, molecular and pathological tests. Most isolates allowed the verification of Koch's postulate on P. pyrifolia seedlings and on slices from immature pear (Pyrus communis) fruits and were also positive in hypersensitivity tests on tobacco leaves. They showed characteristics common to species in the genus Erwinia, but were different from Erwinia amylovora, the agent of fire blight. A relationship between the novel pathogen and E. amylovora was found in microbiological and serological tests. Both organisms had similar but not identical protein patterns in 2-D gel electrophoresis, and in growth morphology the new pathogen produced colonies on MM2 Cu medium that were mucoid and slightly yellow, compared with the clearly yellow colonies of E. amylovora. No similarity was found in the plasmid profiles, and consequently no PCR signal was obtained with primers from the E, amylovora plasmid pEA29. REP-PCR also produced bands differing for the two organisms.
机译：来自韩国亚洲梨树（Pyrus pyrifolia）的坏死分支的细菌始终在营养琼脂上被分离为白色菌落，并在带有硫酸铜的基本培养基上形成粘液状，淡黄色菌落。在一系列微生物学，分子和病理学测试中研究了具有这种菌落形态的分离株。大多数分离株可以验证Pochyrifia幼苗和未成熟梨（Pyrus communis）果实的切片上的Koch假设，并且在烟草叶的超敏反应测试中也呈阳性。它们显示了欧文氏菌属物种共有的特征，但与火疫病的病原体欧文氏菌不同。在微生物学和血清学测试中发现了新型病原体和支链淀粉菌之间的关系。两种生物在2-D凝胶电泳中具有相似但不完全相同的蛋白质模式，并且在生长形态上，新病原体在MM2 Cu培养基上产生的菌落为粘液状且略带黄色，与淀粉状肠球菌的明显黄色菌落相比。在质粒图谱中没有发现相似性，因此用来自淀粉双歧杆菌质粒pEA29的引物没有获得PCR信号。 REP-PCR还产生了两种生物不同的条带。

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