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首页> 外文期刊>Plant Pathology >Prevalence and distribution of Grapevine leaf roll-associated vitus 7 in China detected by an improved reverse transcription polymerase chain reaction assay
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Prevalence and distribution of Grapevine leaf roll-associated vitus 7 in China detected by an improved reverse transcription polymerase chain reaction assay

机译：改进的逆转录聚合酶链反应法检测葡萄叶卷相关病毒7在中国的流行和分布

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摘要

To determine prevalence and distribution of Grapevine leafroll-associated virus 7 (GLRaV-7) in China, a total of 213 grapevine (Vitis spp.) samples (92 popular cultivars) from 13 Chinese provinces and regions were tested for the presence of GLRaV-7 by reverse transcription polymerase chain reaction (RT-PCR) assays and sequencing. GLRaV-7 was found in 40·4% of the samples, and shown to be widely distributed in major grapevine growing areas in China. GLRaV-7 was found in more than 50 grapevine cultivars. Some popular grape cultivars showed a high incidence of GLRaV-7 infection, such as Manicure Finger (100%), Cabernet Sauvignon (83·3%), Merlot (60%), Fujimineri (60%) and Red Globe (50%). The genetic variability of GLRaV-7 isolates was characterized based on partial nucleotide sequences (nucleotides 945-1329 and 97-598 of the ORF) of heat shock protein 70 homologue (HSP70b) and 61-kDa protein (p61) (nucleotides 1073-1572 of the ORF) genes. The overall mean values of nucleotide diversity were low (ranging from 0·009 to 0·066), and phylogenetic analysis based on p61 showed that the GLRaV-7 isolates segregated into three phylogenetic clusters. The results also showed that two previously described primer pairs failed to amplify a wide range of GLRaV-7 isolates present in China. However, a new primer pair reported here, based on the p61 gene and its associated downstream intergenic region, detected more GLRaV-7 Chinese isolates. This is the first report on the prevalence and distribution of GLRaV-7 in China, and also provides an improved RT-PCR assay for detecting the virus.
机译：为了确定中国的葡萄卷叶相关病毒7（GLRaV-7）的流行和分布，对来自中国13个省和地区的213个葡萄（葡萄）样本（92个流行品种）的GLRaV-进行了测试。图7通过逆转录聚合酶链反应（RT-PCR）测定和测序。 GLRaV-7被发现在40·4％的样本中，并被广泛分布在中国主要的葡萄种植区。在超过50个葡萄品种中发现了GLRaV-7。一些受欢迎的葡萄品种表现出较高的GLRaV-7感染率，例如修指甲（100％），赤霞珠（83·3％），梅洛（60％），富士米妮（60％）和红地球（50％） 。基于热休克蛋白70同源物（HSP70b）和61-kDa蛋白（p61）（核苷酸1073-1572）的部分核苷酸序列（ORF的核苷酸945-1329和97-598）表征了GLRaV-7分离株的遗传变异性。 （ORF）基因。核苷酸多样性的总体平均值较低（范围从0·009到0·066），并且基于p61的系统发育分析表明GLRaV-7分离株分为三个系统发育簇。结果还表明，先前描述的两个引物对不能扩增中国存在的多种GLRaV-7分离株。但是，根据p61基因及其相关的下游基因间区域，本文报道了一对新的引物，可检测到更多的GLRaV-7中国分离株。这是关于GLRaV-7在中国流行和分布的第一份报告，也提供了一种改进的RT-PCR检测方法来检测该病毒。

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