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首页> 外文期刊>Plant Pathology >An isolate morphologically and phylogenetically distinct from Botrytis cinerea obtained from withered grapes possibly represents a new species of Botrytis
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An isolate morphologically and phylogenetically distinct from Botrytis cinerea obtained from withered grapes possibly represents a new species of Botrytis

机译：从枯萎的葡萄中分离出的形态和系统发育不同于灰葡萄孢的分离株可能代表了新的葡萄孢属

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摘要

Grapes kept after harvest for withering in drying fruit rooms (fruttaio) are highly susceptible to Botrytis cinerea infection. A taxonomically undefined isolate (B83) was identified during a survey of B.cinerea populations in these specific environments. This isolate yielded negative results in B.cinerea-specific PCRs and displayed a different colony morphology on potato dextrose agar. Based on this data, the isolate underwent a taxonomic investigation. Sequencing of the ITS-58S-ITS2 region indicated that the isolate belongs to the Botrytis genus. IGS-RFLP analysis revealed that Botrytis sp. B83 was a different haplotype from genotypes of indigenous B.cinerea strains. Comparative sequence analysis of three housekeeping genes, G3PDH, HSP60 and RPB2, showed that the isolate constitutes a taxon related to Botrytis aclada, distant from B.cinerea. A phylogenetic tree built with NEP genes was in agreement with the combined tree built with G3PDH, HSP60 and RPB2 sequences. Botrytis sp. B83 is morphologically similar to B.aclada. Conversely, its host range indicates that it is polyphagous like B.cinerea, despite its lower virulence. This study provides evidence for the existence of a new species of Botrytis. Nevertheless, the description of only a single isolate and the need for further information on genetic divergence, reproductive isolation and host preference, prevent the classification and formal description of a new taxon.
机译：收获后保存在干燥的果室（fruttaio）中枯萎的葡萄极易受到灰霉菌的感染。在这些特定环境中对灰葡萄双歧杆菌种群进行调查期间，鉴定出了未分类的分离株（B83）。该分离物在灰葡萄双歧杆菌特异性PCR中产生阴性结果，并且在马铃薯葡萄糖琼脂上显示出不同的菌落形态。基于此数据，对分离株进行了分类研究。 ITS-58S-ITS2区域的测序表明，该分离物属于葡萄孢属。 IGS-RFLP分析表明，葡萄孢属。 B83是不同于本地B.cinerea菌株的基因型的单倍型。对三个管家基因G3PDH，HSP60和RPB2的比较序列分析表明，该分离物构成了与灰葡萄孢菌（B.cinerea）远缘的葡萄孢菌相关的分类单元。用NEP基因构建的系统发育树与用G3PDH，HSP60和RPB2序列构建的组合树一致。葡萄孢属B83在形态上类似于B.aclada。相反，它的寄主范围表明它具有强食性，如灰质芽孢杆菌，尽管其毒性较低。这项研究为新的葡萄孢菌的存在提供了证据。然而，仅对单个分离株的描述以及对遗传多样性，生殖分离和寄主偏好的进一步信息的需求阻止了新分类单元的分类和正式描述。

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