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首页> 外文期刊>Plant Pathology Journal >Complete Nucleotide Sequence of Tomato Leaf Curl Karnataka Virus and p Satellite Molecule Associated with Leaf Curl Disease on Sunflower in India
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Complete Nucleotide Sequence of Tomato Leaf Curl Karnataka Virus and p Satellite Molecule Associated with Leaf Curl Disease on Sunflower in India

机译：印度向日葵叶卷毛卡纳塔克病毒和p卫星分子与叶卷毛病相关的完整核苷酸序列

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Sunflower leaf curl disease caused by begomovirus is one of the newly emerging viral diseases in Nothern Karnataka, Southern India. The disease has attracted lot of attention of the pathologists, as it affects the productivity of sunflower an important oilseed crop in the country. The present study was conducted to characterize the begomovirus causing sunflower leaf curl disease by cloning and sequencing the major genomic components. The Complete DNA-A and betasatellite components of begomovirus isolated from sunflower leaf tissue infected with leaf curl virus collected from Main Agricultural Research Station, Raichur, Karnataka, India was cloned and sequenced. The virus contained the DNA-A and the associated satellite beta DNA components of 2761 and 1373 nucleotides (nt) in length, respectively. The DNA-A molecule shared maximum identity with tomato leaf curl karnataka virus clone IKH12 (ToLCKV-[IKH12]) (HM803118) (97.13%) and clone IKB3 (ToLCKV-[IKJ33]) (HM851186) (96.95%) from India. The betasatellite shared a high nucleotide identity (93.6-94.07%) with Potato apical leaf curl betasatellite (PoLCB-[IN-CHI: 05]) and Papaya leaf curl betasatellite (PaLCuB-[IN-CHI:05]) associated with tomato leaf curl disease from India. The results indicate for the first time that a strain of ToLCKV together with beta DNA satellite causing sunflower leaf curl disease (SuLCD) in India.
机译：由begomovirus引起的向日葵卷叶病是印度南部北卡纳塔克邦新出现的病毒性疾病之一。该病影响了向日葵（该国重要的油料作物）的生产力，因此引起了病理学家的广泛关注。进行本研究以通过克隆和测序主要基因组成分来鉴定引起向日葵叶片卷曲病的贝莫病毒。从从印度卡纳塔克邦赖库尔市主要农业研究站收集的，被叶片卷曲病毒感染的向日葵叶片组织中分离出的begomovirus的完整DNA-A和β卫星组分被克隆并测序。该病毒分别包含2761和1373个核苷酸（nt）的DNA-A和相关的卫星βDNA成分。 DNA-A分子与印度的番茄卷叶卡纳塔克病毒克隆IKH12（ToLCKV- [IKH12]）（HM803118）（97.13％）和克隆IKB3（ToLCKV- [IKJ33]）（HM851186）（96.95％）具有最大同一性。 β卫星与与马铃薯叶相关的马铃薯顶端叶卷曲β卫星（PoLCB- [IN-CHI：05]）和木瓜叶卷曲β卫星（PaLCuB- [IN-CHI：05]）具有高核苷酸同一性（93.6-94.07％）来自印度的卷曲病。结果首次表明，ToLCKV菌株与βDNA卫星一起在印度引起向日葵叶片卷曲病（SuLCD）。

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