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首页> 外文期刊>Plant Pathology Journal >Knockout of ituD Gene of Bacillus subtilis S44 Strain and Impact of its Biocontrol Effect to Cotton Rhizoctoniosis
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Knockout of ituD Gene of Bacillus subtilis S44 Strain and Impact of its Biocontrol Effect to Cotton Rhizoctoniosis

机译：枯草芽孢杆菌S44菌株ituD基因的敲除及其对棉花根瘤菌病防治作用的影响

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摘要

Bacillus subtilis S44 is a promising plant growth-promoting rhizobacterium and a potent biocontrol agent isolated from cotton rhizosphere. The ituD gene was cloned and identified from S44. A suicide vector containing ituD gene from B. subtilis S44 wasrecombined into the B. subtilis S44 chromosome to disrupt the ituD gene. Recombination was demonstrated by PCR, antifungal activity and HPLC confirmed the loss of the ituD gene. The growth rate of the recombinant is severely depressed compared with wildtype and tetracycline had no effect on the growth rate the mutant strains. Antifungal activity in cell-free extracts of the recombinant has almost no antifungal activity compared with the wild strain. HPLC analysis show that the wild strian produce iturin A at an amount of 89.4 ug mL~(-1) while the ituD mutant only 3.41 pg mL~(-1). In pot and plot tests, the control effects of S44 treatment were 80.30 and 72.97%, respectively. The mutant strains SI and S2 exhibit no control effect of cotton Rhizoctoniosis. These results suggest that the ituD gene was important for iturin A synthesis and biocontrol activity of S44.
机译：枯草芽孢杆菌S44是一种有前途的植物促生根瘤菌，也是一种从棉花根际分离的有效生物防治剂。从S44克隆并鉴定ituD基因。将包含来自枯草芽孢杆菌S44的ituD基因的自杀载体重组到枯草芽孢杆菌S44染色体中以破坏ituD基因。通过PCR证实了重组，抗真菌活性和HPLC证实了ituD基因的丢失。与野生型相比，重组体的生长速度严重降低，四环素对突变菌株的生长速度没有影响。与野生菌株相比，重组体的无细胞提取物中的抗真菌活性几乎没有抗真菌活性。 HPLC分析表明，野生链产生的尿素A的量为89.4ug mL〜（-1），而ituD突变体仅为3.41pg mL〜（-1）。在盆栽试验中，S44处理的防治效果分别为80.30和72.97％。突变株S1和S2没有表现出对棉花根瘤菌病的控制作用。这些结果表明，ituD基因对于Iurin A的合成和S44的生物防治活性很重要。

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