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首页> 外文期刊>Plant Pathology Journal >Pseudomonas fluorescens Induced Enzymological Changes in Banana Roots (Cv. Rasthali) against Fusarium Wilt Disease
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Pseudomonas fluorescens Induced Enzymological Changes in Banana Roots (Cv. Rasthali) against Fusarium Wilt Disease

机译：荧光假单胞菌诱导香蕉根（Cv。Rasthali）抗枯萎病的酶学变化

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摘要

Pseudomonas fluorescens isolated from banana rhizosphere reduced the vascular discolouration associated with Fusarium wilt disease and induced the accumulation of resistance associated enzymes in roots. The banana roots inoculated with talc based formulation of P. fluorescens at 10 g / plant had minimum vascular discolouration index when compared to pathogen Fusarium oxysporum f.sp. cubense alone inoculated roots. The resistance associated enzymes viz, peroxidase and polyphenol oxidase activity increased two folds on 8 days in roots treated with P. fluorescens and challenged with F oxysporum f.sp. cubense Whereas phenylalanine ammonia lyase activity increased two folds on 6 days, later shows decreasing trend in the roots. In F. oxysporum f.sp. cubense alone inoculated plants, peroxidase, polyphenol oxidase and phenylalanine ammonia lyase activities increased upto 4 days after inoculation. The induction was compared with other fungal biocontrol agent's viz. Trichoderma viride and Trichoderma harzianum. Same trend of enzymes induction were noticed in T. viride and T. harzianum inoculated roots. Further more, in Native Poly Acrlyamide Gel Electrophoresis analysis, two isoforms of peroxidase and five isofbrms of polyphenol oxidase were observed in roots treated with P. fluorescens and challenged with F. oxysporum f.sp. cubense.
机译：从香蕉根际分离的荧光假单胞菌减少了与枯萎病相关的血管变色，并诱导了根系中抗性相关酶的积累。当与致病性尖孢镰刀菌（Fusarium oxysporum f.sp.）相比时，以滑石粉为基础的荧光假单胞菌制剂以10 g /株接种的香蕉根具有最小的血管变色指数。单独接种立方体的根。抗性相关的酶即过氧化物酶和多酚氧化酶活性在8天后在荧光假单胞菌和oxy porsporum f.sp处理的根中增加了2倍。立方体中苯丙氨酸解氨酶活性在6天时增加了2倍，后来在根部显示出下降的趋势。在F.oxysporum f.sp.中。单独接种立方体植物后，接种后长达4天，过氧化物酶，多酚氧化酶和苯丙氨酸氨裂解酶的活性增加。将诱导与其他真菌生物防治剂的作用进行比较。绿色木霉和哈茨木霉。在T. viride和T. harzianum接种的根中发现了相同的酶诱导趋势。此外，在天然聚丙烯酰胺凝胶电泳分析中，在用荧光假单胞菌处理并用氧化假单胞菌（F.oxysporum f.sp.）攻击的根中观察到过氧化物酶的两种同工型和多酚氧化酶的五个同工型。立方。

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