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首页> 外文期刊>Plant Growth Regulation: An International Journal on Natural and Synthetic Regulators >In vitro organogenesis from leaf and transverse thin cell layer derived callus cultures of Talinum triangulare (Jacq.) Willd.
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In vitro organogenesis from leaf and transverse thin cell layer derived callus cultures of Talinum triangulare (Jacq.) Willd.

机译：从三角叶um（Jacq。）Willd的叶片和横向薄细胞层衍生的愈伤组织培养物中进行体外器官发生。

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摘要

Talinumtriangulare is an important medicinal herb used traditionally in the treatment of various diseases. The present study was intended to develop a rapid and efficient protocol for indirect organogenesis from leaf discs and transverse thin cell layer (tTCL) of internodal explants of T. triangulare. Best callusing response (100 %) was observed with tTCL explants on Murashige and Skoog (MS) medium supplemented with 4.44 μM 6-benzyl amino purine and 5. 37 μM α-naphthalene acetic acid (NAA). High frequency shoot regeneration (96. 67 %) was obtained from tTCL derived calli on MS medium supplemented with a combination of 0.45 μM thidiazuron and 0. 27 μM NAA, by producing 9. 20 ± 0.35 shoots with a shoot length of 2.74 ± 0. 03 cm. In vitro rooting of the microshoots was recorded on half-strength MS medium containing 2. 46 μM indole-3-butyric acid by eliciting 15.20 ± 0.27 roots with a length of 4. 25 ± 0. 11 cm. The rooted shoots were acclimatized on garden soil, sand and coco pith (1:1:3 v/v) planting substrate. The plantlets were successfully established under field conditions with 100 % survival rate. The hardened plants exhibited homogeneity and no observable morphological variations were detected among the regenerants and the mother plants of T. triangulare.
机译：三角藻是传统上用于治疗各种疾病的重要药用植物。本研究旨在开发一种快速有效的方案，用于从三角盘茎结节外植体的叶盘和横向薄细胞层（tTCL）间接进行器官发生。在补充了4.44μM6-苄基氨基嘌呤和5. 37μMα-萘乙酸（NAA）的Murashige和Skoog（MS）培养基上，使用tTCL外植体观察到最佳的回拨响应（100％）。在补充了0.45μM噻唑隆和0. 27μMNAA的MS培养基上，从tTCL衍生的愈伤组织获得了高频芽再生（96. 67％），产生了9个。20±0.35个芽，芽长为2.74±0 03厘米通过激发15.20±0.27根长为4. 25±0. 11 cm的根，在含有2. 46μM吲哚-3-丁酸的半强度MS培养基上记录微枝的生根。使生根的芽适应于花园土壤，沙子和可可豆（1：1：3 v / v）的种植基质上。在田间条件下以100％成活率成功建立了幼苗。硬化的植物表现出同质性，并且在三角锥虫的再生体和母本植物之间未发现可观察到的形态变化。

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