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首页> 外文期刊>Biomaterials >Directed cell attachment by tropoelastin on masked plasma immersion ion implantation treated PTFE.
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Directed cell attachment by tropoelastin on masked plasma immersion ion implantation treated PTFE.

机译：原弹性蛋白指导细胞附着在经掩蔽的等离子体浸没离子注入处理的PTFE上。

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The ability to generate cell patterns on polymer surfaces is critical for the detailed study of cellular biology, the fabrication of cell-based biosensors, cell separation techniques and for tissue engineering. In this study contact tape masking and steel shadow masks were used to exclude plasma immersion ion implantation (PIII) treatment from defined areas of polytetrafluoroethylene (PTFE) surfaces. This process enabled patterned covalent binding of the cell adhesive protein, tropoelastin, without employing chemical linking molecules. Tropoelastin coating rendered the untreated regions cell adhesive and the PIII-treated area non-adhesive, allowing very fine patterning of cell adhesion to PTFE surfaces. A blocking step, such as with BSA or PEG, was not required to prevent cell binding to the underlying PIII-treated regions as tropoelastin coating alone performed this blocking function. Although tropoelastin coated the entire PTFE surface, the cell binding C-terminus of tropoelastin was markedly less solvent exposed on the PIII-treated, hydrophilic regions. The differential exposure of the C-terminus correlated with the patterned distribution of tropoelastin-mediated cell adhesion. This new methodology specifically enables directed cell behavior on a polymer surface using a simple one-step treatment process, by modulating the adhesive activity of a single extracellular matrix protein.
机译：在聚合物表面产生细胞模式的能力对于细胞生物学的详细研究，基于细胞的生物传感器的制造，细胞分离技术以及组织工程至关重要。在这项研究中，使用接触带掩膜和钢制荫罩从聚四氟乙烯（PTFE）表面的限定区域中排除了等离子体浸没离子注入（PIII）处理。此过程无需使用化学连接分子即可实现细胞粘附蛋白原弹性蛋白的图案化共价结合。 Tropoelastin涂层使未处理的区域具有细胞粘附性，而PIII处理的区域则没有粘附性，从而使细胞对PTFE表面的粘附性非常精细。不需要封闭步骤（例如使用BSA或PEG）来阻止细胞与下面的PIII处理区域结合，因为仅原弹性蛋白涂层即可执行此封闭功能。尽管原弹性蛋白覆盖了整个PTFE表面，但原弹性蛋白的细胞结合C端在PIII处理的亲水性区域上暴露的溶剂少得多。 C末端的差异暴露与原弹性蛋白介导的细胞粘附的模式分布相关。通过调节单个细胞外基质蛋白的黏附活性，这种新的方法可以通过简单的一步处理过程，使定向的细胞行为直接在聚合物表面发生。

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