Detection of heterologous bovine pancreatic trypsin inhibitor by capillary zone electrophoresis

Galli A.; Ghezzi L.; Raspi G.; Secco F.; Spinetti M.

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Detection of heterologous bovine pancreatic trypsin inhibitor by capillary zone electrophoresis

机译：毛细管区带电泳检测异源牛胰胰蛋白酶抑制剂

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A method based on the use of capillary zone electrophoresis (CZE) is proposed for the determination of the heterologous protein bovine pancreatic trypsin inhibitor (BPTI) expressed in yeast. Saccharomyces cerevisiae cells were transformed with plasmid pCB235U and the amount of inhibitor expressed in yeast was measured both in the culture medium (supernatant) and the cell extract. Supernatant samples were investigated by the CZE method preceded by affinity chromatography (the BPTI yield was between 2 and 5 mug ml(-1)); direct determination of BPTI in cell extract was performed taking advantage of the complex formation of BPTI and trypsin (BPTI yield was 5 mug ml(-1)). (C) 2002 Elsevier Science Ltd. All rights reserved. [References: 16]

机译：提出了一种基于毛细管区带电泳（CZE）的方法，用于测定酵母中表达的异源蛋白牛胰胰蛋白酶抑制剂（BPTI）。用质粒pCB235U转化酿酒酵母细胞，并在培养基（上清液）和细胞提取物中测量酵母中表达的抑制剂的量。上清液样品先通过CZE方法进行研究，然后进行亲和层析（BPTI产量在2至5毫升ml（-1）之间）;利用BPTI和胰蛋白酶的复杂形成来直接测定细胞提取物中的BPTI（BPTI产量为5杯ml（-1））。（C）2002 Elsevier ScienceLtd。保留所有权利。 [参考：16]

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《Polyhedron: The International Journal for Inorganic and Organometallic Chemistry》 |2002年第15期|共6页
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Galli A.; Ghezzi L.; Raspi G.; Secco F.; Spinetti M.;
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1. Detection of heterologous bovine pancreatic trypsin inhibitor by capillary zone electrophoresis [J] . Galli A., Ghezzi L., Raspi G., Polyhedron: The International Journal for Inorganic and Organometallic Chemistry . 2002,第14a15期

机译：毛细管区带电泳检测异源牛胰胰蛋白酶抑制剂
2. The influence of bovine pancreatic trypsin inhibitor hydrophobization on ability to inhibit bovine trypsin and human leukocyte elastase [J] . E. V. Malykh, O. P. Tyurina, N. G. Balabushevitch Биохимия . 1998,第10期

机译：牛胰胰蛋白酶抑制剂疏水化对抑制牛胰蛋白酶和人白细胞弹性酶的能力的影响
3. Structure of single-disulfide variants of bovine pancreatic trypsin inhibitor (BPTI) as probed by their binding to bovine beta-trypsin [J] . Krokoszynska I., Otlewski J., Dadlez M. Journal of Molecular Biology . 1998,第3期

机译：牛胰胰蛋白酶抑制剂（BPTI）的单二硫键变体与牛β-胰蛋白酶结合的结构
4. Synthetic approach to study the effects of beta-turn residues on the structure and folding of bovine pancreatic trypsin inhibitor (BPTI) [C] . Judit Tulla, Clear Woodward, George Barany the International Peptide Symposium . 2001

机译：研究β转残渣对牛胰胰蛋白酶抑制剂结构和折叠的影响的合成方法（BPTI）
5. ELECTROSTATIC INTERACTIONS AND ION BINDING IN TRYPSIN, BOVINE PANCREATIC TRYPSIN INHIBITOR, AND OTHER PROTEINS (STABILITY, PROTON). [D] . MARCH, KEITH LEONARD. 1983

机译：胰蛋白酶，牛胰胰蛋白酶抑制剂和其他蛋白质（稳定性，质子）中的静电相互作用和离子结合。
6. Two-dimensional capillary electrophoresis: capillary isoelectric focusing and capillary zone electrophoresis with laser-induced fluorescence detection [O] . Jane A. Dickerson, Lauren M. Ramsay, Oluwatosin O. Dada, -1

机译：二维毛细管电泳：毛细管等电聚焦和毛细管区带电泳用激光诱导的荧光检测
7. Bovine pancreatic trypsin inhibitor-trypsin complex as a detection system for recombinant proteins. [O] . Borjigin, J, Nathans, J 1993

机译：牛胰胰蛋白酶抑制剂-胰蛋白酶复合物作为重组蛋白的检测系统。

Detection of heterologous bovine pancreatic trypsin inhibitor by capillary zone electrophoresis

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