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首页> 外文期刊>Polyhedron: The International Journal for Inorganic and Organometallic Chemistry >Synthesis, characterization, DNA interaction and cleavage activity of new mixed ligand copper(II) complexes with heterocyclic bases
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Synthesis, characterization, DNA interaction and cleavage activity of new mixed ligand copper(II) complexes with heterocyclic bases

机译：具有杂环碱基的新型混合配体铜（II）配合物的合成，表征，DNA相互作用和裂解活性

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摘要

Mixed ligand complexes having the formulae Cu(RPO)(2)PY2, Cu(RPO)(2)Im(2), and Cu(DBO)(2)Py-2 [RPO = resacetophenone oxime, DBO = 2,4-dihydroxybenzophenone oxime, Py = pyridine and Ina = imidazole] have been synthesized and characterized by UV-Vis, IR, ESR, cyclic voltammetry and magnetic susceptibility methods. Absorption studies revealed that each of these octahedral complexes is an avid binder of calf thymus DNA. The apparent binding constants for mixed ligand complexes are in order of 10(4)-10(5) M-1. Based on the data obtained in the DNA binding studies a partial intercalative mode of binding is suggested for these complexes. The nucleolytic cleavage activity of the adducts was carried out on double stranded pBR322 circular plasmid DNA by using a gel electrophoresis experiment in the presence and absence of oxidant (H2O2). All the metal complexes cleaved supercoiled DNA by hydrolytic and oxidative paths. The oxidative path dominates the hydrolytic cleavage. The hydrolytic cleavage of DNA is evidenced from the control experiments showing discernable cleavage inhibition in the presence of the hydroxyl radical inhibitor DMSO or the singlet oxygen quencher azide ion. (c) 2006 Elsevier Ltd. All rights reserved.
机译：具有式Cu（RPO）（2）PY2，Cu（RPO）（2）Im（2）和Cu（DBO）（2）Py-2的混合配体配合物[RPO =对苯二甲酰肟，DBO = 2,4-合成了二羟基二苯甲酮肟，Py =吡啶，Ina =咪唑]，并通过UV-Vis，IR，ESR，循环伏安法和磁化率方法进行了表征。吸收研究表明，这些八面体复合物中的每一种都是小牛胸腺DNA的狂热结合物。混合配体复合物的表观结合常数约为10（4）-10（5）M-1。根据在DNA结合研究中获得的数据，建议对这些复合物进行部分插入结合。在存在和不存在氧化剂（H 2 O 2）的情况下，通过凝胶电泳实验在双链pBR322环状质粒DNA上进行加合物的核酸裂解活性。所有的金属配合物都通过水解和氧化途径切割超螺旋DNA。氧化途径主导水解裂解。对照实验证明了DNA的水解裂解，在羟基自由基抑制剂DMSO或单线态氧猝灭叠氮离子的存在下，其裂解抑制作用明显。 （c）2006 Elsevier Ltd.保留所有权利。

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