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首页> 外文期刊>Physiology and Molecular Biology of Plants >Characterization of seed storage proteins in high protein genotypes of cowpea [Vigna unguiculata (L.) Walp.]
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Characterization of seed storage proteins in high protein genotypes of cowpea [Vigna unguiculata (L.) Walp.]

机译：cow豆高蛋白基因型种子贮藏蛋白的表征[Vigna unguiculata（L.）Walp。]

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Twenty one genotypes and two check varieties viz. CS-88 and V-240 of cowpea [Vigna unguiculata (L.) Walp. ] were screened for total proteins. The total protein content ranged from 22.4 (HC-3) to 27.9 % (HC-98-64) in 21 genotypes whereas in check varieties it was 25.6 (V-240) and 26.0 % (CS-88). Seven genotypes viz. HC-6, HC-5, CP-21, LST-II-C-12, CP-16, COVU-702 and HC-98-64 having high protein content (26.7 to 27.9 %) were selected for further characterization of their seed storage proteins. Globulins were the major protein fraction ranging from 55.6 (LST-II-C-12) to 58.8 % (CP-16 and HC-6) of total protein. Glutelins was the second major fraction ranging from 14.4 to 15.6 % followed by albumins (8.2 to 11.9 %) and prolamins (2.3 to 5.0 %). Content of free amino acids also showed variations amongst genotypes with COVU-702 having maximum and LST-II-C-12 having minimum content. Essential amino acid analysis revealed that S-amino acids (cysteine and methionine) were the first limiting amino acids followed by tryptophan. From the results presented here it could be suggested that two genotypes viz. LST-II-C-12 and HC-5 be used in breeding programmes aimed at developing high protein moth bean varieties with good quality.
机译：21个基因型和2个检查品种。 cow豆的CS-88和V-240 [Vigna unguiculata（L.）Walp。筛选总蛋白。在21个基因型中，总蛋白含量从22.4（HC-3）到27.9％（HC-98-64）不等，而在对照品种中，总蛋白含量为25.6（V-240）和26.0％（CS-88）。七个基因型。选择具有高蛋白含量（26.7％至27.9％）的HC-6，HC-5，CP-21，LST-II-C-12，CP-16，COVU-702和HC-98-64进行进一步表征种子贮藏蛋白。球蛋白是主要蛋白部分，占总蛋白的55.6（LST-II-C-12）至58.8％（CP-16和HC-6）。谷蛋白是第二主要部分，范围从14.4％至15.6％，其次是白蛋白（8.2％至11.9％）和谷醇溶蛋白（2.3％至5.0％）。游离氨基酸的含量在基因型之间也表现出差异，其中COVU-702含量最高，而LST-II-C-12含量最低。必需氨基酸分析显示，S-氨基酸（半胱氨酸和蛋氨酸）是第一个限制性氨基酸，其次是色氨酸。从这里给出的结果可以暗示两个基因型。 LST-II-C-12和HC-5可用于旨在开发优质高蛋白蛾豆品种的育种计划。

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