Multivariate analysis and statistical mixture designs were used for chromatographic fingerprint preparation and authentication of the plant material of three species of the genus Bauhinia. The extracts were analysed by reversed-phase high-performance liquid chromatography. Mixture design gave an optimum solvent composition for extracting components from the plants of 36% dichloromethane, 17% ethanol and 47% ethyl acetate (by volume), while an optimum mobile phase for chromatographic analyses was found to be 27% methanol, 27% acetonitrile and 46% of water (by volume). Results from principal component analysis, hierarchical analysis and soft independent modelling by class analogy showed that Bauhinia candicans cannot be synonymous with B. forficata Link. It was also possible to trace the metabolic profile without identifying its chemical constituents and to determine a chromatographic discriminating region. The characteristics responsible for discrimination between B. candicans and B. forficata were more polar substances that presented peaks with retention times around 1.65 and 1.81 min. Copyright pb 2007 John Wiley & Sons, Ltd.
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机译：多变量分析和统计混合物设计用于色谱指纹图谱的制备和三种紫荆属植物材料的鉴定。通过反相高效液相色谱分析提取物。混合物设计给出了从植物中提取成分的最佳溶剂组成，该成分为36％二氯甲烷，17％乙醇和47％乙酸乙酯（按体积计），而色谱分析的最佳流动相为27％甲醇，27％乙腈和46％的水（按体积计）。通过类比的主成分分析，层次分析和软独立建模的结果表明，紫荆花不能与B. forficata Link成为同义词。也有可能在不鉴定其化学成分的情况下追踪代谢曲线，并确定色谱区分区域。区分B. candicans和B. forficata的特征是极性较大的物质，其峰出现的保留时间约为1.65和1.81 min。版权所有pb 2007 John Wiley＆Sons，Ltd.
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