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首页> 外文期刊>Physiology and Molecular Biology of Plants >Plant regeneration in Chlorophytum borivilianum Sant. et Fernand. from embryogenic callus and cell suspension culture and assessment of genetic fidelity of plants derived through somatic embryogenesis.
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Plant regeneration in Chlorophytum borivilianum Sant. et Fernand. from embryogenic callus and cell suspension culture and assessment of genetic fidelity of plants derived through somatic embryogenesis.

机译：绿生吊兰植物再生。 et Fernand。从胚性愈伤组织和细胞悬浮培养中提取，并通过体细胞胚发生评估植物的遗传保真度。

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摘要

Efficient in vitro propagation of medicinally important endangered plant C. borivilianum has been achieved through somatic embryogenesis. Solid embryogenic medium [Murashige and Skoog medium containing 1.79 mM NH4NO3, 10.72 mM KNO3, 1.13 micro M 2,4-dichlorophenoxyacetic acid, 7.38 micro M 2-isopentenyladenine and 0.76 mM proline] supplemented with polyethylene glycol and sucrose (3% each), exhibited 1.88-fold increase in embryo maturation compared to embryogenic medium containing 3% sucrose. Liquid embryogenic medium supported better somatic embryo production and maturation. Highest total (79) and mature (cotyledonary stage) somatic embryos (38) as well as highest germination (57.5%) was observed at inoculum density of 0.4 g/40 ml of liquid medium. 5.86 pH level exhibited optimal growth, maturation and germination of somatic embryos. Random amplified polymorphic DNA (RAPD) analysis of C. borivilianum plants regenerated through somatic embryogenesis revealed that they were genetically similar to the mother plant. The protocol established in the present study can be used for rapid mass multiplication of C. borivilianum in bioreactor employing liquid medium.CAS Registry Numbers 9007-49-2 25322-68-3 147-85-3 57-50-1
机译：通过体细胞胚发生已经实现了具有医学重要性的濒危植物C. borivilianum的有效体外繁殖。固体胚发生培养基[Murashige and Skoog培养基，含有1.79 mM NH 4 NO 3 ，10.72 mM KNO 3 ，1.13 micro M 2,4-二氯苯氧基乙酸与含3％蔗糖的胚发生培养基相比，添加了聚乙二醇和蔗糖（每种3％）的硫酸，7.38微M 2-异戊烯腺嘌呤和0.76 mM脯氨酸]的胚成熟度提高了1.88倍。液体胚发生培养基支持更好的体细胞胚产生和成熟。在0.4 g / 40 ml液体培养基的接种密度下，观察到最高的总数（79）和成熟的（子叶期）体细胞胚（38）以及最高的发芽率（57.5％）。 pH值5.86表现出最佳的体细胞胚生长，成熟和萌发状态。通过体细胞胚发生再生的C. borivilianum植物的随机扩增多态DNA（RAPD）分析表明，它们在遗传上与母本相似。本研究建立的方案可用于在使用液体培养基的生物反应器中快速进行牛毛衣梭菌的大规模繁殖.CAS登记号9007-49-2 25322-68-3 147-85-3 57-50-1

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