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首页> 外文期刊>Physiology and Molecular Biology of Plants >Cloning and characterization of cold, salt and drought inducible C-repeat binding factor gene from a highly cold adapted ecotype of Lepidium latifolium L.
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Cloning and characterization of cold, salt and drought inducible C-repeat binding factor gene from a highly cold adapted ecotype of Lepidium latifolium L.

机译：高度适应寒冷的生态型小叶小叶锦鸡儿的冷，盐和干旱诱导的C-重复结合因子基因的克隆和鉴定。

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摘要

The dehydration-responsive element-binding (DREB) protein/C-Repeat Binding Factors (CBFs) belongs to APETALA2 (AP2) family transcription factors that binds to DRE/CRT cis-element in cold-responsive (COR) genes and induce COR genes. CBFs have been isolated and characterized from evolutionarily diverse plait species. CBF pathway is conserved by CBF regulon and the size or the number and kind of target genes vary among freezing sensitive and tolerant plants. Hence, cloning of CBFs from highly freezing tolerant plants such as Lepidium latifolium L. will be useful in understanding the freezing tolerance of this species. In this study, LICBF, a CBF1 family gene fromX. Latifolium L., was cloned using RT-PCR and RACE-PCR. The Ml length mRNA of LICBF is 948bp with an open reading frame of 642 bp, encoding a protein of 213 amino acids with a molecular weight of 23.92 kDa and a theoretical isoelectric point of 4.80. Amino acid sequence analysis showed that LICBF has an AP2 DNA binding domain, a potential CBFtype nuclear localization signal (NLS) aid C-terminal acidic domain. Semi-quantitative RT-PCR analysis of LICBF revealed that this gene is up-regulated by high salt, dehydration and low temperature stresses. The investigation is therefore successful incloning of a gene having strong homology with CBF transcription factors and responsive to low temperature, high salt and dehydration conditions.
机译：脱水反应元件结合（DREB）蛋白/ C重复结合因子（CBF）属于APETALA2（AP2）家族转录因子，与冷反应（COR）基因中的DRE / CRT顺式元件结合并诱导COR基因。 CBFs已从进化不同的辫状物种中分离和鉴定。 CBF通路由CBF调节子保守，靶基因的大小或数量和种类在冷冻敏感和耐性植物之间有所不同。因此，从高度耐寒的植物，如小叶小叶菊（Lepidium latifolium L.）克隆CBF将有助于理解该物种的耐寒性。在这项研究中，LICBF是X的CBF1家族基因。使用RT-PCR和RACE-PCR克隆乳杆菌。 LICBF的M1长度mRNA为948bp，开放阅读框为642bp，编码213个氨基酸的蛋白质，分子量为23.92kDa，理论等电点为4.80。氨基酸序列分析表明LICBF具有一个AP2 DNA结合域，一个潜在的CBF型核定位信号（NLS）辅助C端酸性域。 LICBF的半定量RT-PCR分析表明，该基因在高盐，脱水和低温胁迫下被上调。因此，这项研究成功地克隆了与CBF转录因子具有高度同源性并响应低温，高盐和脱水条件的基因。

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