• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Phytochemical Analysis >Separation of Four Homoisoflavonoids from Caesalpinia sappan byTI Separation of Four Homoisoflavonoids from Caesalpinia sappan by High-speed Counter-current Chromatography
【24h】

Separation of Four Homoisoflavonoids from Caesalpinia sappan byTI Separation of Four Homoisoflavonoids from Caesalpinia sappan by High-speed Counter-current Chromatography

机译：TI从TI子中分离出四种同黄酮类化合物的高速逆流色谱法分离从Ca子中分离出四种同黄酮类化合物

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Introduction - The main chemical constituents of Caesalpinia sappan are homoisoflavonoids. Conventional column chromatographic techniques used for isolation of this type of compounds are tedious, time-consuming and waste solvents. High-speed counter-current chromatography (HSCCC) could be a suitable alternative for the enrichment and purification of these target compounds from traditional Chinese medicine (TCM). Objective - To establish a method to isolate four homoisoflavonoids in one-step separation from C. sappan by HSCCC. Methodology - The crude extract of C. sappan was fractionated by HSCCC using a two-phase solvent system consisting of chloroform-methanol-water (4:3:2, v/v/v). The separation conditions were:flow rate, 1.0mL/min; revolution speed, 900 rpm; detection wavelength, 280 nm; separation temperature, 25 degrees C; sample size, 120 mg crude sample dissolved in a mixture of the upper and lower phases (10 mL each). The retention of the stationary phase was 83%. Results - Five milligrams of 3'-deoxysappanol, 8 mg of 3-deoxysappanone B, 20 mg of 4-O-methylsappanol and 18 mg of brazilin were obtained in one-step separation from 120 mg of an ethyl acetate extracted fraction of C. sappan. Their purities were 99%, 97%, 90% and 85% by HPLC analysis. The mean recoveries of the four compounds were 83%, 86%, 93% and 85%, respectively. Conclusion - The study has shown that HSCCC is effective for the separation and enrichment of the target compounds at a large scale
机译：简介-凯撒pin的主要化学成分是同异黄酮。用于分离这类化合物的常规柱色谱技术是乏味，费时且浪费的溶剂。高速逆流色谱（HSCCC）可能是从中药（TCM）富集和纯化这些目标化合物的合适替代方法。目的-建立一种通过HSCCC一步法分离蓝C中四种同型异黄酮的方法。方法-用由氯仿-甲醇-水（4：3：2，v / v / v）组成的两相溶剂系统，通过HSCCC对粗制的C. sappan提取物进行分馏。分离条件为：流速1.0mL / min。转速900 rpm;检测波长280 nm;分离温度25℃;样品大小，将120 mg粗样品溶解在上，下相的混合物中（每相10 mL）。固定相的保留率为83％。结果-从120 mg乙酸乙酯萃取的C馏分中一步分离获得5毫克3'-脱氧沙巴醇，8 mg 3-脱氧沙巴酮B，20 mg 4-O-甲基沙巴醇和18 mg巴西林。萨潘通过HPLC分析，它们的纯度为99％，97％，90％和85％。四种化合物的平均回收率分别为83％，86％，93％和85％。结论-研究表明，HSCCC可以有效地大规模分离和富集目标化合物

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号