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首页> 外文期刊>Phytochemical Analysis >HPLC fingerprints combined with principal component analysis, hierarchical cluster analysis and linear discriminant analysis for the classification and differentiation of Peganum sp. indigenous to China
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HPLC fingerprints combined with principal component analysis, hierarchical cluster analysis and linear discriminant analysis for the classification and differentiation of Peganum sp. indigenous to China

机译：HPLC指纹图谱与主成分分析，层次聚类分析和线性判别分析相结合，用于对Peganum sp。进行分类和区分。中国原住民

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摘要

Introduction - Seeds of wild Peganum harmala Linn., P. multisectum (Maxim) Bobr., P. nigellastrum Bunge and a probable indeterminate species, herein referred to as P. variety, are commonly used in Chinese medicine. These seeds cannot be differentiated based on morphology.Objective - Seeds of P. harmala Linn., P. multisectum (Maxim) Bobr., P. nigellastrum Bunge and P. variety were collected in different provinces in China and their HPLC profiles were recorded for statistical analysis and pattern recognition.Methodology - HPLC chromatograms of seed extracts were recorded under the same conditions. Individual HPLC chromatograms for each species were evaluated against the mean chromatogram for the same species generated using a similarity evaluation computer program. Data from chromatographic fingerprints were also processed using principal component analysis (PCA), hierarchical cluster analysis (HCA) and linear discriminant analysis (LDA).Results - The Peganum sp. seed extracts had similar HPLC fingerprints but with some inter-specific differences. The chromatographic fingerprints combined with PCA, HCA and LDA could distinguish the seeds of the different species of Peganum investigated.Conclusion - HPLC fingerprints can be used to authenticate and differentiate the seeds of three different species of genus Peganum indigenous to China. The results indicated that the unidentified P. variety might indeed be a new species or variety
机译：引言-野生Peganum harmala Linn。，P。multisectum（Maxim）Bobr。，P。nigellastrum Bunge和可能的不确定物种的种子，在本文中被称为P.变种。目的-在中国不同省份收集哈密瓜，多裂豆，黑僵菌和种的种子，并记录其HPLC图谱。统计分析和模式识别。方法学-在相同条件下记录种子提取物的HPLC色谱图。使用相似性评估计算机程序，将每个物种的单个HPLC色谱图与相同物种的平均色谱图进行比较。色谱指纹图谱的数据也使用主成分分析（PCA），层次聚类分析（HCA）和线性判别分析（LDA）处理。种子提取物具有相似的HPLC指纹图谱，但存在一些种间差异。色谱指纹图谱与PCA，HCA和LDA结合可以区分所研究的不同品种的Peganum种子。结论-HPLC指纹图谱可用于鉴定和区分中国本土的三种不同Peganum属种子。结果表明，未鉴定的P.品种确实可能是新物种或新品种。

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