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首页> 外文期刊>Phytochemical Analysis >In-tube Extraction and GC-MS Analysis of Volatile Components from Wild and Cultivated sea buckthorn (Hippophae rhamnoides L ssp. Carpatica) Berry Varieties and Juice
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In-tube Extraction and GC-MS Analysis of Volatile Components from Wild and Cultivated sea buckthorn (Hippophae rhamnoides L ssp. Carpatica) Berry Varieties and Juice

机译：野生和栽培沙棘浆果沙棘品种和汁中挥发性成分的管内提取和GC-MS分析

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Introduction - The health benefits of sea buckthorn {Hippophae rhamnoides L.) are well documented due to its rich content in bioactive phytochemicals (pigments, phenolics and vitamins) as well as volatiles responsible for specific flavours and bacteriostatic action. The volatile compounds are good biomarkers of berry freshness, quality and authenticity. Objective - To develop a fast and efficient GC-MS method including a minimal sample preparation technique (in-tube extraction, ITEX) for the discrimination of sea buckthorn varieties based on their chromatographic volatile fingerprint. Material and methods - Twelve sea buckthorn varieties (wild and cultivated) were collected from forestry departments and experimental fields, respectively. The extraction of volatile compounds was performed using the ITEX technique whereas separation and identification was performed using a GC-MS QP-2010. Principal component analysis (PCA) was applied to discriminate the differences among sample composition. Results -Using GC-MS analysis, from the headspace of sea buckthorn samples, 46 volatile compounds were separated with 43 being identified. The most abundant derivatives were ethyl esters of 2-methylbutanoic acid, 3-methylbutanoic acid, hexanoic acid, octanoic acid and butanoic acid, as well as 3-methylbutyl 3-methylbutanoate, 3-methylbutyl 2-methylbutanoate and benzoic acid ethyl ester (over 80% of all volatile compounds). Principal component analysis showed that the first two components explain 79% of data variance, demonstrating a good discrimination between samples. Conclusion - A reliable, fast and eco-friendly ITEX/GC-MS method was applied to fingerprint the volatile profile and to discriminate between wild and cultivated sea buckthorn berries originatingfrom the Carpathians, with relevance to food science and technology.
机译：简介-沙棘（Hippophae rhamnoides L.）的健康益处已得到充分证明，原因是沙棘中含有丰富的生物活性植物化学成分（色素，酚类和维生素）以及负责特定风味和抑菌作用的挥发物。挥发性化合物是浆果新鲜度，质量和真实性的良好生物标记。目的-开发一种快速高效的GC-MS方法，该方法包括基于样品挥发性色谱指纹图谱鉴别沙棘品种的最低限度样品制备技术（试管内提取，ITEX）。材料和方法-分别从林业部门和试验场收集了十二个沙棘品种（野生和栽培）。挥发性化合物的提取使用ITEX技术进行，而分离和鉴定则使用GC-MS QP-2010进行。应用主成分分析（PCA）来区分样品成分之间的差异。结果-使用GC-MS分析，从沙棘样品的顶空分离出46种挥发性化合物，其中43种已被鉴定出来。最丰富的衍生物是2-甲基丁酸，3-甲基丁酸，己酸，辛酸和丁酸的乙酯，以及3-甲基丁酸3-甲基丁酯，2-甲基丁酸3-甲基丁酯和苯甲酸乙酯（所有挥发性化合物的80％）。主成分分析表明，前两个成分可解释79％的数据差异，表明样本之间有很好的区分度。结论-一种可靠，快速且生态友好的ITEX / GC-MS方法用于指纹图谱的挥发性特征，并区分与喀尔巴阡山脉相关的野生沙棘果和栽培沙棘果，并与食品科学和技术相关。

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