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首页> 外文期刊>Polymers for advanced technologies >The biodegradation of poly(3-hydroxy-butyrate-co-3-hydroxyvalerate) (PHB/V and PHB/V-degrading microorganisms in soil)
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The biodegradation of poly(3-hydroxy-butyrate-co-3-hydroxyvalerate) (PHB/V and PHB/V-degrading microorganisms in soil)

机译:聚(3-羟基丁酸酯-co-3-羟基戊酸酯)的生物降解(PHB / V和PHB / V降解土壤中的微生物)

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摘要

To assess the polyhydroxyalkanoate (PHA)-biodegrading capacity of soil, numbers of aerobic poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB/V)-degrading microorganisms (degraders) were estimated by the film-MPN method. The numbers of PHBJV degraders were estimated to be 4.3 × 10{sup}5 per gram of dry garden soil, 5.06 × 10{sup}5 per gram of dry paddy-field soil, and 3.87 × 10{sup}5 per gram of dry river-bank soil. The degradations of PHB/V in suspensions of the three kinds of soil were investigated. It was found that the PHB/V-biodegrading capacity of the soil increased as the number of PHB/V degraders in the soil increased. The relationship between weight loss of PHB/V and proliferation rate of PHB/V degraders in garden soil suspension was investigated. The effect of glucose on the biodegradation of PHBIV in garden soil was also studied. The weight loss after one week in garden soil suspension supplemented with 20 mM of glucose (GSS-20G) was 2.60%, which was lower than that in garden soil suspension (GSS) (7.14%). After five weeks, the weight loss had increased to 24.97% in GSS-20G but only to 18.26% in GSS. The results showed that glucose played important roles in inhibition and acceleration of different biodegrading phases and finally accelerated the PHB/V biodegradation in soil suspension.
机译:为了评估土壤中的聚羟基链烷酸酯(PHA)的生物降解能力,通过膜MPN方法估算了需氧的降解聚(3-羟基丁酸酯-co-3-羟基戊酸酯)(PHB / V)的微生物(降解剂)的数量。 PHBJV降解物的数量估计为每克旱花园土壤4.3×10 {sup} 5,每克旱田土壤5.06×10 {sup} 5和3.87×10 {sup} 5每克干燥的河岸土壤。研究了三种土壤悬浮液中PHB / V的降解情况。发现随着土壤中PHB / V降解物的数量增加,土壤的PHB / V生物降解能力增加。研究了土壤土壤悬浮液中PHB / V的失重与PHB / V降解剂增殖速率之间的关系。还研究了葡萄糖对菜园土壤中PHBIV生物降解的影响。补充了20 mM葡萄糖的花园土壤悬浮液(GSS-20G)一周后的重量损失为2.60%,低于花园土壤悬浮液(GSS)(7.14%)的重量损失。五周后,GSS-20G的体重减轻增加到24.97%,而GSS的增加只有18.26%。结果表明,葡萄糖在不同生物降解阶段的抑制和促进中起着重要作用,最终促进了土壤悬浮液中PHB / V的生物降解。

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